Usui E, Noshiro M, Ohyama Y, Okuda K
Department of Biochemistry, Hiroshima University School of Dentistry, Japan.
FEBS Lett. 1990 Nov 12;274(1-2):175-7. doi: 10.1016/0014-5793(90)81357-t.
The cDNA for vitamin D 25-hydroxylase in rat liver mitochondria was transfected in COS cells in order to confirm our previous postulation that both 5 beta-cholestane-3 alpha, 7 alpha, 12 alpha-triol 27-hydroxylation and vitamin D 25-hydroxylation are catalyzed by a common enzyme. As a result it was found that both enzyme activities could be reconstituted from the solubilized extract of mitochondria of these cells, NADPH, NADPH-adrenodoxin reductase and adrenodoxin, giving unequivocal evidence that the two enzyme activities are catalyzed by a common enzyme.
为了证实我们之前的假设，即5β-胆甾烷-3α,7α,12α-三醇27-羟化和维生素D 25-羟化均由一种共同的酶催化，将大鼠肝脏线粒体中维生素D 25-羟化酶的cDNA转染到COS细胞中。结果发现，这两种酶活性都可以从这些细胞的线粒体、NADPH、NADPH-肾上腺皮质铁氧化还原蛋白还原酶和肾上腺皮质铁氧化还原蛋白的可溶提取物中重建，这明确证明了这两种酶活性是由一种共同的酶催化的。
