鳄梨（Persea americana）细胞色素 P-450 与单萜类化合物的相互作用。
Interactions of Avocado (Persea americana) Cytochrome P-450 with Monoterpenoids.
机构信息
Biochemistry and Physiology Department, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, Harpenden, Herts. AL5 2JQ, United Kingdom.
出版信息
Plant Physiol. 1992 Apr;98(4):1290-7. doi: 10.1104/pp.98.4.1290.
Abstract
The microsomal fraction of avocado (Persea americana) mesocarp is a rich source of cytochrome P-450 active in the demethylation of xenobiotics. Cytochrome P-450 from this tissue has been purified and well characterized at the molecular level (DP O'Keefe, KJ Leto [1989] Plant Physiol 89: 1141-1149; KR Bozak, H Yu, R Sirevag, RE Christoffersen [1990] Proc Natl Acad Sci USA 87: 3904-3908). Despite this extensive characterization, the role of the enzyme in vivo was not established. Optical and electron paramagnetic resonance binding studies described here suggest that the monoterpenoids, nerol and geraniol, are substrates of avocado cytochrome P-450 (spectral dissociation constant of 7.2 and 35 micromolar, respectively). Avocado microsomes have been shown to catalyze the hydroxylation of these monoterpenoids, and both nerol and geraniol have been shown to inhibit the activity of avocado cytochrome P-450 toward the artificial substrate 7-ethoxycoumarin, with nerol a competitive inhibitor of this activity.
摘要
鳄梨（Persea americana）中果皮的微粒体部分是细胞色素 P-450 的丰富来源，该酶在异源生物的去甲基化中起作用。这种组织中的细胞色素 P-450 已在分子水平上得到纯化和很好的表征（DP O'Keefe，KJ Leto [1989] Plant Physiol 89: 1141-1149; KR Bozak，H Yu，R Sirevag，RE Christoffersen [1990] Proc Natl Acad Sci USA 87: 3904-3908）。尽管有了广泛的特征描述，但该酶在体内的作用尚未确定。本文描述的光学和电子顺磁共振结合研究表明，单萜类化合物橙花醇和香叶醇是鳄梨细胞色素 P-450 的底物（光谱离解常数分别为 7.2 和 35 微摩尔）。鳄梨微粒体已被证明可以催化这些单萜类化合物的羟化作用，橙花醇和香叶醇都被证明可以抑制鳄梨细胞色素 P-450 对人工底物 7-乙氧基香豆素的活性，橙花醇是这种活性的竞争性抑制剂。
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