Tikhomirova N K, Merchan A Y, Kochetov G A
A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.
FEBS Lett. 1990 Nov 12;274(1-2):27-9. doi: 10.1016/0014-5793(90)81321-e.
Using an immunosorbent, a new form of transketolase, namely, an enzyme-RNA complex, was isolated from a baker's yeast extract. Spontaneous fission of RNA (or its enzymic hydrolysis by RNase) is accompanied by a sharp increase in the catalytic activity of transketolase, which may be directly related to the enzyme's regulation mechanism.
利用免疫吸附剂,从面包酵母提取物中分离出一种新形式的转酮醇酶,即一种酶 - RNA复合物。RNA的自发裂变(或其被核糖核酸酶的酶促水解)伴随着转酮醇酶催化活性的急剧增加,这可能与该酶的调节机制直接相关。