Tikhomirova N K, Kochetov G A
Biokhimiia. 1991 Jun;56(6):1123-30.
A new procedure for the isolation of homogeneous transketolase from baker's yeast based on the use of enzyme-specific antibodies immobilized on a insoluble matrix has been developed. The enzyme yield is 90% of its total content in the original yeast extract. The eluate from the immunocolumn was found to contain a previously unknown form of transketolase which represents an enzyme-RNA complex.
基于使用固定在不溶性基质上的酶特异性抗体,已开发出一种从面包酵母中分离纯一同工转酮醇酶的新方法。酶产量为原始酵母提取物中其总含量的90%。发现免疫柱洗脱液中含有一种以前未知的转酮醇酶形式,它代表一种酶-RNA复合物。