Moreira Samara S J, Silva Andréia M, Pereira Ana G, Santos Romário P, Dantas Maiko R T, Souza-Júnior João B F, Snoeck Paola P N, Silva Alexandre R
Laboratory of Animal Germplasm Conservation, Department of Animal Sciences, Federal University of Semiarid Region-UFERSA, Mossoró 59625-900, Brazil.
Laboratory of Animal Reproduction, State University of Santa Cruz-UESC, Ilhéus 45662-900, Brazil.
Animals (Basel). 2023 Jan 28;13(3):451. doi: 10.3390/ani13030451.
We evaluated the effects of detergents based on sodium dodecyl sulfoxide (SDS) on the functional parameters of collared peccary frozen-thawed sperm. Semen aliquots from ten individuals were diluted in a Tris-egg yolk-glycerol extender alone or with 0.5% Equex STM paste or SDS (at 0.1%, 0.3% or 0.5% () concentration). Samples were fast frozen in liquid nitrogen with a post-thaw evaluation of motility, membrane functionality and integrity, mitochondrial activity, sperm binding ability and thermal resistance. The treatments without SDS (41.8 ± 3.5%) and those containing Equex (41.8 ± 4.4%) or 0.1% SDS (41.2 ± 5.5%) provided greater sperm motility ( < 0.05) than those containing SDS 0.3% (30.5 ± 4.7%) and 0.5% (31.2 ± 6.3%). Immediately after thawing, only treatments containing 0.1% SDS effectively preserved sperm straightness (STR) when compared to the negative control. All treatments preserved the amplitude of lateral head (ALH) and straightness (STR) during a thermal resistance test ( > 0.05), but SDS 0.5% impaired the membrane functionality and mitochondrial activity after thawing ( < 0.05). All treatments provided a similar recovery of sperm binding ability after thawing ( < 0.05). Our results showed that the addition of 0.1% SDS to the Tris-yolk-glycerol extender optimized the freeze-thaw recovery of peccary semen.
我们评估了基于十二烷基硫氧化物（SDS）的去污剂对领西貒冻融精子功能参数的影响。从十只个体采集的精液等分试样分别单独用Tris-蛋黄-甘油稀释剂稀释，或与0.5%的Equex STM糊剂或SDS（浓度分别为0.1%、0.3%或0.5%）混合稀释。样品在液氮中快速冷冻，并在解冻后评估其活力、膜功能与完整性、线粒体活性、精子结合能力和热抗性。不含SDS的处理组（41.8±3.5%）以及含有Equex的处理组（41.8±4.4%）或0.1% SDS的处理组（41.2±5.5%）的精子活力高于含有0.3% SDS（30.5±4.7%）和0.5% SDS（31.2±6.3%）的处理组（P<0.05）。解冻后立即观察发现，与阴性对照组相比，仅含有0.1% SDS的处理组能有效保持精子的直线度（STR）。在热抗性测试中，所有处理组均能保持侧向头部摆动幅度（ALH）和直线度（STR）（P>0.05），但0.5% SDS处理组在解冻后损害了膜功能和线粒体活性（P<0.05）。解冻后所有处理组的精子结合能力恢复情况相似（P<0.05）。我们的结果表明，在Tris-蛋黄-甘油稀释剂中添加0.1% SDS可优化领西貒精液的冻融回收率。
