Faculty of Biotechnology, University of Wrocław, Przybyszewskiego 63/77, 51-148 Wroclaw, Poland.
J Agric Food Chem. 2012 May 16;60(19):5003-12. doi: 10.1021/jf300421m. Epub 2012 May 7.
The composition of polyunsaturated fatty acids in the tissues is very important to human health and strongly depends on dietary intake. Since flax seeds are the richest source of polyunsaturated acids, their consumption might be beneficial for human health. Unfortunately, they are highly susceptible to auto-oxidation, which generates toxic derivatives. The main goal of this study was the generation of genetically modified flax plants with increased antioxidant potential and stable and healthy oil production. Since among phenylpropanoid compounds those belonging to the flavonoid route have the lowest antioxidant capacity, the approach was to inhibit this route of the pathway, which might result in accumulation of other compounds more effective in antioxidation. The suppression of the chalcone synthase gene resulted in hydrolyzable tannin accumulation and thus increased antioxidant status of seeds of the transgenic plant. This was due to the partial redirecting of substrates for flavonoid biosynthesis to the other routes of the phenylpropanoid pathway. Consequently, transgenic plants produced more (20-45%) polyunsaturated fatty acids than the control and mainly α-linolenic acid. Thus, increasing the antioxidant potential of flax plants has benefits in terms of the yield of suitable oil for human dietary consumption.
多不饱和脂肪酸在组织中的组成对人类健康非常重要，并且强烈依赖于饮食摄入。由于亚麻籽是多不饱和酸最丰富的来源，因此它们的消费可能对人类健康有益。不幸的是，它们极易自动氧化，产生有毒衍生物。本研究的主要目标是生成具有增强的抗氧化潜力和稳定健康的油生产的转基因亚麻植物。由于在苯丙素化合物中，属于类黄酮途径的化合物具有最低的抗氧化能力，因此方法是抑制该途径，这可能导致更有效的抗氧化化合物的积累。查尔酮合酶基因的抑制导致可水解单宁的积累，从而增加了转基因植物种子的抗氧化状态。这是由于类黄酮生物合成的底物部分重新定向到苯丙素途径的其他途径。因此，与对照相比，转基因植物产生了更多的（20-45％）多不饱和脂肪酸，主要是α-亚麻酸。因此，提高亚麻植物的抗氧化能力在适合人类食用的油的产量方面具有益处。
