Instituto de Tecnologia Química e Biológica-Universidade Nova de Lisboa, 2780-901 Oeiras, Portugal.
Trends Biotechnol. 2012 Jul;30(7):377-85. doi: 10.1016/j.tibtech.2012.03.004. Epub 2012 Apr 26.
A central concern of biopharmaceutical R&D is the production of sufficient quantities of recombinant products from manufacturing processes based on animal cell culture. The way in which bioprocess researchers have addressed this question experienced a tremendous shift over the years, progressing from almost empirical to more rational approaches. A step further is the application of systems biotechnology: recent technological advances for large-scale cell state characterization and creative methods for host cell modeling are becoming crucial for next-generation bioprocess optimization. Here we provide an overview of the main trends towards this goal, with a focus on metabolic models as central scaffolds for data integration and prediction of bioprocess outcomes.
生物制药研发的一个核心关注点是如何在基于动物细胞培养的生产工艺中，大量生产重组产品。多年来，生物工艺研究人员一直在解决这个问题，他们的方法经历了从几乎凭经验到更具理性的巨大转变。更进一步的是系统生物技术的应用：用于大规模细胞状态特征描述的最新技术进步和宿主细胞建模的创造性方法，对于下一代生物工艺优化变得至关重要。在这里，我们概述了实现这一目标的主要趋势，重点介绍了代谢模型作为数据集成和生物工艺结果预测的核心支架。
