Pakyz R E, Heindel R M, Kallish M, Cosentino M J
Department of Biology, Millersville University, Millersville, Pennsylvania 17551.
J Androl. 1990 Sep-Oct;11(5):401-8.
Unilateral spermatic cord torsion results in contralateral degeneration and reduced fertility in the prepubertal male rat. This study was conducted to investigate the use of immunosuppression with cyclosporine and prednisone to prevent these untoward effects. Thirty-five-day-old male rats were subjected to 720 degrees unilateral spermatic cord torsion of 9 hours duration. At the time of detorsion, animals were given a subcutaneous injection of i) cyclosporine, ii) prednisone, or iii) cyclosporine combined with prednisone. Control groups included: i) animals undergoing orchiectomy of the ipsilateral testis following the torsion period, ii) hemicastration in the absence of torsion and iii) sham surgery. Orchiectomy at the end of the torsion period prevented the torsion induced reduction of fertility, contralateral seminiferous tubule diameter and testis weight. Treatment with cyclosporine combined with prednisone significantly increased these parameters above detorsion alone. These data indicate that short term immunosuppression with cyclosporine alone or in combination with prednisone limits the adverse effects of unilateral spermatic cord torsion as does removal of the damaged organ at the end of the torsion period.
单侧精索扭转会导致青春期前雄性大鼠对侧睾丸退化并降低生育能力。本研究旨在探讨使用环孢素和泼尼松进行免疫抑制以预防这些不良影响。对35日龄雄性大鼠进行持续9小时的720度单侧精索扭转。在扭转解除时，给动物皮下注射：i）环孢素，ii）泼尼松，或iii）环孢素联合泼尼松。对照组包括：i）扭转期后行同侧睾丸切除术的动物，ii）未扭转情况下的半侧睾丸切除术，以及iii）假手术。扭转期末行睾丸切除术可防止扭转引起的生育能力降低、对侧曲细精管直径减小和睾丸重量减轻。环孢素联合泼尼松治疗显著提高了这些参数，使其高于单纯扭转解除后的水平。这些数据表明，单独使用环孢素或与泼尼松联合进行短期免疫抑制，与扭转期末切除受损器官一样，可限制单侧精索扭转的不良影响。
