Wen Yan-Lin, Deng Xian-Zhong, Yang Zheng-Wei, Wang An-Guo, Zhang Zong-Ping, Wu Ji, Jiang Tao, Tang Shuo, Cai Yun-Lin, Li Yun-Xiang, Fan Jun
Department of Urology, The Second School of Clinical Medicine, The Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan 637000, China.
Zhonghua Nan Ke Xue. 2013 Jul;19(7):604-11.
To investigate the protective effect of retarded removal of the unilateral necrotic testis after long-time (> 24 h) spermatic cord torsion on the contralateral testis in rats.
Thirty-three male SD rats aged 21 -42 days were divided into a sham-operation group (n = 11), a torsion-reservation group (n = 12) and a torsion-orchiectomy group (n = 10). The rats of the sham-operation group received dartos pouch orchidopexy on the left testis, while those of the latter two groups underwent 720 degrees unilateral spermatic cord torsion on the left side. Ninety-six hours later, the rats of the torsion-reservation group received detorsion with the ipsilateral testis preserved, while those of the torsion-orchiectomy group underwent orchiectomy. Three months after operation, blood samples were obtained from the rats for measurement of serum testosterone and antisperm antibodies by ELISA, and meanwhile testes and epididymides were harvested for determination of the volumes of various structures and the diameter of seminiferous tubules with stereological methods.
There were no significant differences in the level of serum testosterone among the three groups. Anti-sperm antibody positive was found in only 1 animal in the torsion-reservation group. The Leydig cell nuclei in the contralateral testis appeared larger in the torsion groups than in the sham-operation group. Marked morphological changes were observed in 1, 3 and 0 of the animals in the sham-operation, torsion-reservation and torsion-orchiectomy group, respectively, mainly including atrophy of seminiferous tubules and reduced number of spermatogenic cells. The volume of the contralateral testis was increased by 19% and 21% in the torsion-reservation and torsion-orchiectomy group, respectively, in comparison with that in the sham-operation group (P < 0.05). No significant differences were observed in the volume of seminiferous tubules of the contralateral testis among the sham-operation, torsion-reservation and torsion-orchiectomy groups ([1.15 +/- 0.07], [1.30 +/- 0.04] and [1.35 +/- 0.05] cm3). The volume of the interstitial tissue was significantly increased in the latter two groups ([0.36 +/- 0.02 and 0.34 +/- 0.03] cm3) as compared with the former ([0.25 +/- 0.02] cm3) (P < 0.05). The diameters of the seminiferous tubules exhibited no significant differences among the three groups ([226.00 +/- 7.00], [223.00 +/- 6.00] and [221.00 +/- 3.0] microm).
Long-time unilateral spermatic cord torsion may result in compensatory hypertrophy of the contralateral testis, and orchiectomy does not significantly affect the histology of the contralateral testis and epididymis.
探讨长时间（>24小时）精索扭转后延迟切除单侧坏死睾丸对大鼠对侧睾丸的保护作用。
将33只21 - 42日龄雄性SD大鼠分为假手术组（n = 11）、扭转保留组（n = 12）和扭转睾丸切除组（n = 10）。假手术组大鼠行左侧睾丸肉膜囊固定术，后两组大鼠行左侧720度单侧精索扭转。96小时后，扭转保留组大鼠行扭转复位并保留同侧睾丸，扭转睾丸切除组大鼠行睾丸切除术。术后3个月，采集大鼠血液样本，采用酶联免疫吸附测定法（ELISA）检测血清睾酮和抗精子抗体，同时摘取睾丸和附睾，采用体视学方法测定各结构体积和生精小管直径。
三组大鼠血清睾酮水平无显著差异。扭转保留组仅1只动物抗精子抗体呈阳性。扭转组对侧睾丸的Leydig细胞核比假手术组的大。假手术组、扭转保留组和扭转睾丸切除组分别有1只、3只和0只动物出现明显形态学改变，主要包括生精小管萎缩和生精细胞数量减少。扭转保留组和扭转睾丸切除组对侧睾丸体积分别比假手术组增加19%和21%（P < 0.05）。假手术组、扭转保留组和扭转睾丸切除组对侧睾丸生精小管体积无显著差异（[1.15 ± 0.07]、[1.30 ± 0.04]和[1.35 ± 0.05] cm³）。后两组间质组织体积显著增加（[0.36 ± 0.02和0.34 ± 0.03] cm³），而假手术组为（[0.25 ± 0.02] cm³）（P < 0.05）。三组生精小管直径无显著差异（[226.00 ± 7.00]、[223.00 ± 6.00]和[221.00 ± 3.0] µm）。
长时间单侧精索扭转可能导致对侧睾丸代偿性肥大，睾丸切除对侧睾丸和附睾的组织学无显著影响。
