采用快速实时聚合酶链反应检测呼吸道临床标本中的肺炎支原体和肺炎衣原体。
Detection of Mycoplasma pneumoniae and Chlamydophila pneumoniae directly from respiratory clinical specimens using a rapid real-time polymerase chain reaction assay.
机构信息
Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
出版信息
Diagn Microbiol Infect Dis. 2012 Jul;73(3):278-80. doi: 10.1016/j.diagmicrobio.2012.03.024. Epub 2012 Apr 25.
Abstract
We developed a rapid real-time polymerase chain reaction assay for detecting Mycoplasma pneumoniae and Chlamydophila pneumoniae directly from respiratory specimens. This procedure provides over 5 times faster results compared to existing methods while maintaining equivalent detection rates for specimens containing limited target organisms.
摘要
我们开发了一种快速实时聚合酶链反应检测方法，可直接从呼吸道标本中检测肺炎支原体和肺炎衣原体。与现有方法相比，该方法的结果快 5 倍以上，同时对于包含有限目标生物的标本保持等效的检测率。
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