一种优化的用于 ZFN 介导的基因靶向的双指文库。
An optimized two-finger archive for ZFN-mediated gene targeting.
机构信息
Program in Gene Function and Expression, University of Massachusetts Medical School, Worcester, USA.
出版信息
Nat Methods. 2012 Jun;9(6):588-90. doi: 10.1038/nmeth.1994. Epub 2012 Apr 29.
The widespread use of zinc-finger nucleases (ZFNs) for genome engineering is hampered by the fact that only a subset of sequences can be efficiently recognized using published finger archives. We describe a set of validated two-finger modules that complement existing finger archives and expand the range of ZFN-accessible sequences threefold. Using this archive, we introduced lesions at 9 of 11 target sites in the zebrafish genome.
锌指核酸酶（ZFNs）在基因组工程中的广泛应用受到限制，因为只有一小部分序列可以使用已发布的指纹档案进行有效识别。我们描述了一组经过验证的双指纹模块，这些模块补充了现有的指纹档案，并将 ZFN 可访问的序列范围扩大了三倍。使用这个档案，我们在斑马鱼基因组的 11 个目标位点中的 9 个引入了损伤。
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