黑曲霉 DLFCC-90 鼠李糖苷水解酶，一种新型的黄酮糖苷水解酶。
Aspergillus niger DLFCC-90 rhamnoside hydrolase, a new type of flavonoid glycoside hydrolase.
机构信息
College of Biotechnology, Dalian Polytechnic University, China.
出版信息
Appl Environ Microbiol. 2012 Jul;78(13):4752-4. doi: 10.1128/AEM.00054-12. Epub 2012 Apr 27.
Abstract
A novel rutin-α-L-rhamnosidase hydrolyzing α-L-rhamnoside of rutin, naringin, and hesperidin was purified and characterized from Aspergillus niger DLFCC-90, and the gene encoding this enzyme, which is highly homologous to the α-amylase gene, was cloned and expressed in Pichia pastoris GS115. The novel enzyme was classified in glycoside-hydrolase (GH) family 13.
摘要
从黑曲霉 DLFCC-90 中纯化并鉴定了一种新型芦丁-α-L-鼠李糖苷酶，可水解芦丁、柚皮苷和橙皮苷的α-L-鼠李糖苷。克隆并在毕赤酵母 GS115 中表达了与α-淀粉酶基因高度同源的该酶基因。该新酶被分类为糖苷水解酶（GH）家族 13。
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