Diabetes Research Institute, Miller School of Medicine, University of Miami, Miami, Florida 33136, USA.
J Biomed Mater Res A. 2012 Aug;100(8):1963-71. doi: 10.1002/jbm.a.34052. Epub 2012 Apr 28.
Molecules of pentadecafluorooctanoyl chloride (PFC) were grafted onto alginate (Alg) using a linear poly(ethylene glycol) linker and amide bonds. The resulting Alg-PFC material was characterized by proton nuclear magnetic resonance and infrared spectroscopies. The degree of PFC functionalization significantly influenced the physical and chemical properties of Alg-PFC, particularly when the resulting polymer was ionically crosslinked into hydrogels. Alg-PFC hydrogel beads fabricated via Ba(2+) crosslinking were found to match the permeability properties of control alginate beads, except upon swelling over time in culture media. When used to encapsulate MIN6 cells, a beta cell line, Alg-PFC beads demonstrated enhanced cell proliferation over alginate control beads. These results indicate that Alg-PFC hydrogels retain some of the PFC's biological-relevant benefits, such as enhancement of mass transport and bioinertness, to enhance cellular viability within alginate three-dimensional hydrogel environments. We envision these functionalized hydrogels to be particularly useful in the encapsulation of cells with a high metabolic demand, such as pancreatic islets.
使用线性聚乙二醇（PEG）连接物和酰胺键，将十五氟辛酰氯（PFC）的分子嫁接到藻酸盐（Alg）上。通过质子核磁共振和红外光谱对所得的Alg-PFC 材料进行了表征。PFC 官能度的程度显著影响 Alg-PFC 的物理和化学性质，特别是当所得聚合物离子交联成水凝胶时。通过 Ba(2+)交联制备的 Alg-PFC 水凝胶珠在培养基中随时间膨胀时，与对照藻酸盐珠的渗透性相匹配。当用于包封 MIN6 细胞（β细胞系）时，Alg-PFC 珠表现出比藻酸盐对照珠更高的细胞增殖能力。这些结果表明，Alg-PFC 水凝胶保留了一些与 PFC 相关的生物学益处，例如增强质量传递和生物惰性，以提高藻酸盐三维水凝胶环境中细胞的活力。我们设想这些功能化水凝胶在封装高代谢需求的细胞（如胰岛）方面特别有用。
