Department of Chemical and Biomolecular Engineering, 4 Engineering Drive 4, National University of Singapore, 117576, Singapore.
Small. 2012 Aug 6;8(15):2421-9. doi: 10.1002/smll.201102353. Epub 2012 Apr 30.
Herein is reported the one-step synthesis of an integrated nanocomposite with eccentrically loaded 5 nm gold nanoparticles (Au NPs) and conjugated polymer of poly[9,9-bis(6'-N,N,N-trimethylammonium)hexyl)fluorenyldivinylene-alt-4,7-(2,1,3,- benzothiadiazole) dibromide] (PFVBT). The nanocomposite is generated with surface-functionalized folic acid groups due to the matrix polymer of PLGA-PEG(2000) -folate used for encapsulation. The nanocomposite shows far-red fluorescence from PFVBT and scattering signal from Au NPs. Although Au NPs have been widely reported to quench the fluorescence of conjugated polymers, the PFVBT fluorescence is well maintained in the nanocomposite due to the eccentric location of Au NPs. The folic acid groups at the nanocomposite surface favor its cellular uptake by MCF-7 breast cancer cells, which have overexpressed folate receptors on the cell membranes. In conjugation with its low cytotoxicity, the folic-acid-functionalized nanocomposite has been successfully utilized for fluorescence and dark-field dual-modal targeted cellular imaging.
本文报道了一种一步法合成的纳米复合材料，其中负载有偏心的 5nm 金纳米粒子（Au NPs）和共轭聚合物聚[9,9-双（6'-N，N，N-三甲基铵）己基芴基二乙烯基并恶二唑]（PFVBT）。由于用于封装的 PLGA-PEG（2000）-叶酸基质聚合物具有表面功能化的叶酸基团，因此生成了纳米复合材料。该纳米复合材料显示出来自 PFVBT 的远红荧光和来自 Au NPs 的散射信号。尽管 Au NPs 已被广泛报道会猝灭共轭聚合物的荧光，但由于 Au NPs 的偏心位置，PFVBT 的荧光在纳米复合材料中得到了很好的保持。纳米复合材料表面的叶酸基团有利于其被细胞膜上过表达叶酸受体的 MCF-7 乳腺癌细胞摄取。结合其低细胞毒性，叶酸功能化的纳米复合材料已成功用于荧光和暗场双模态靶向细胞成像。
