Wan Xiaofang, Liu Maixian, Ma Mingze, Chen Danyang, Wu Na, Li Li, Li Zhongjun, Lin Guimiao, Wang Xiaomei, Xu Gaixia
National-Regional Key Technology Engineering Laboratory for Medical Ultrasound, Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Department of Biomedical Engineering, School of Medicine, Shenzhen University, Shenzhen, China.
Key Laboratory of Optoelectronics Devices and Systems of Ministry of Education/Guangdong Province, College of Optoelectronic Engineering, Shenzhen University, Shenzhen, China.
Front Pharmacol. 2019 Feb 26;10:141. doi: 10.3389/fphar.2019.00141. eCollection 2019.
Nanomaterials with localized surface plasmon resonance (LSPR) have exquisite optical properties, which allow a wide range of applications. Non-stoichiometric copper sulfides with active LSPR have drawn great attention, because its LSPR peak falls in the NIR region that is suitable for deep bioimaging and photothermal therapy (PTT). Despite numerous biomedical applications, the biocompatibility and toxicity of copper sulfides have not been studied systematically. In this contribution, we synthesized the ultrasmall biocompatible copper sulfide nanoparticle encapsulated within bovine serum albumin (BSA), CuS@BSA. The physical features of CuS@BSA were characterized. The MTT and flow cytometry assays were performed. The PTT was also investigated. The results indicated that such CuS@BSA nanoparticle had an average TEM size of 8 nm, and an average DLS size of 15 nm. A lower concentration of CuS@BSA was not toxic to HeLa cells, but the critical apoptotic events occurred in HeLa cells after co-incubation with 45 μg/mL CuS@BSA for 48 h. The photothermal effect of the CuS@BSA in aqueous medium were concentration-dependent and time-dependent, which were also verified by flow cytometry and microscopy, while the CuS@BSA were co-cultured with HeLa cells and treated with laser. This work designed an ultrasmall potential biocompatible nanoparticle, CuS@BSA, for cancer photothermal therapy, and provided the toxic information to safely guide its biomedical applications.
具有局域表面等离子体共振（LSPR）的纳米材料具有优异的光学性能，可实现广泛应用。具有活性LSPR的非化学计量硫化铜备受关注，因为其LSPR峰位于适合深度生物成像和光热疗法（PTT）的近红外区域。尽管有众多生物医学应用，但硫化铜的生物相容性和毒性尚未得到系统研究。在本研究中，我们合成了包裹在牛血清白蛋白（BSA）中的超小生物相容性硫化铜纳米颗粒CuS@BSA。对CuS@BSA的物理特性进行了表征。进行了MTT和流式细胞术分析。还研究了PTT。结果表明，这种CuS@BSA纳米颗粒的平均透射电镜尺寸为8nm，平均动态光散射尺寸为15nm。较低浓度的CuS@BSA对HeLa细胞无毒，但在与45μg/mL CuS@BSA共孵育48小时后，HeLa细胞发生了关键的凋亡事件。CuS@BSA在水性介质中的光热效应具有浓度依赖性和时间依赖性，在与HeLa细胞共培养并用激光处理时，流式细胞术和显微镜也证实了这一点。这项工作设计了一种用于癌症光热治疗的超小潜在生物相容性纳米颗粒CuS@BSA，并提供了毒性信息以安全指导其生物医学应用。
