Kesavan Manjula, Kaliaperumal Rajendran, Tamilmani Eevera, Shanmugam Kumaran
Department of Biotechnology, Periyar Maniammai University, Thanjavur, Tamil Nadu, India.
Scand J Urol Nephrol. 2012 Aug;46(4):290-7. doi: 10.3109/00365599.2012.669792. Epub 2012 May 1.
Calcium oxalate monohydrate (COM) crystals are frequently found in urinary calculi (stones).
COM crystals were grown by the single diffusion gel growth technique and the inhibitory effects of aqueous extracts of leaves, stems and rhizome of Costus igneus on the growth of COM crystals were studied.
With an increase in the concentration of aqueous extract of C. igneus, the weight of the formed crystals was gradually reduced from 2.15 to 0.07 g(leaves), 0.06 g (rhizome) and 0.03 g (stem). The crystals harvested from the COM were characterized by Fourier transform infrared spectroscopy to confirm the functional groups and powder X-ray diffraction analyses to confirm the crystalline phases of COM crystals. A scanning electron microscopy study confirmed that the morphology of the crystals changed from hexagonal (COM) to bipyramids of calcium oxalate dihydrate (COD).
This study confirms that using aqueous extracts of stem and rhizome of C. igneus can promote the formation of COD crystals and reduce the nucleation rate of COM crystals, a major component of calcium urinary stones.
一水合草酸钙（COM）晶体常见于尿路结石中。
采用单扩散凝胶生长技术培养COM晶体，并研究了艳山姜叶、茎和根茎水提取物对COM晶体生长的抑制作用。
随着艳山姜水提取物浓度的增加，形成的晶体重量逐渐从2.15克（叶）、0.06克（根茎）和0.03克（茎）减少。通过傅里叶变换红外光谱对从COM收获的晶体进行表征，以确认官能团，并通过粉末X射线衍射分析确认COM晶体的晶相。扫描电子显微镜研究证实，晶体形态从六边形（COM）变为二水合草酸钙（COD）的双锥体。
本研究证实，使用艳山姜茎和根茎的水提取物可促进COD晶体的形成，并降低COM晶体（钙尿路结石的主要成分）的成核速率。
