Kang Seokha, Kim Jiyeon, Lee Junhee, Kim Sanghee, Min Gi-Sik, Park Joong-Ki
Department of Biotechnology and Bioinformatics, College of Science & Technology, Korea University, Chungnam, Republic of Korea.
Mitochondrial DNA. 2012 Jun;23(3):176-8. doi: 10.3109/19401736.2012.668900. Epub 2012 May 1.
An exponential growth of mitochondrial genome information has brought significant progress in understanding the organismal phylogeny and mitochondrial genome evolution for many metazoans including platyhelminth groups. In this study, we determined the complete mitochondrial genome sequence for Benedenia hoshinai, an ectoparasitic monogenean species, and compared it with its congener Benedenia seriolae. The complete mitochondrial genome is 13,554 bp in length and contains 12 protein-coding genes (lacking the atp8 gene), 2 rRNA genes, and 22 tRNA genes, all encoded in the same direction as found in all other platyhelminth species sequenced to date. The gene arrangement of B. hoshinai mtDNA is almost identical to B. seriolae, differing only by the translocation of trnT between cox1 and rrnL. It is unclear whether the shared position of trnT between B. hoshinai and Gyrodactylus represents evidence for their phylogenetic affinity; testing this hypothesis requires further mitogenomic evidence.
线粒体基因组信息呈指数增长，这在理解包括扁形虫类群在内的许多后生动物的系统发育和线粒体基因组进化方面取得了重大进展。在本研究中，我们确定了外寄生单殖吸虫物种星野贝内登虫（Benedenia hoshinai）的完整线粒体基因组序列，并将其与其同属的黄尾贝内登虫（Benedenia seriolae）进行了比较。完整的线粒体基因组长度为13,554 bp，包含12个蛋白质编码基因（缺少atp8基因）、2个rRNA基因和22个tRNA基因，所有基因均按与迄今为止测序的所有其他扁形虫物种相同的方向编码。星野贝内登虫线粒体DNA的基因排列与黄尾贝内登虫几乎相同，仅trnT在cox1和rrnL之间的易位有所不同。尚不清楚星野贝内登虫和三代虫属（Gyrodactylus）之间trnT的共同位置是否代表它们系统发育亲缘关系的证据；检验这一假设需要更多的线粒体基因组证据。
