Department of Veterinary Hygiene, Veterinary School, Nippon Veterinary and Life Science University, 1-7-1 Kyounan, Musashino, Tokyo, 180-8602, Japan.
Vet Res. 2012 Apr 30;43(1):41. doi: 10.1186/1297-9716-43-41.
The feline infectious peritonitis virus (FIPV) is a member of the feline coronavirus family that causes FIP, which is incurable and fatal in cats. Cyclosporin A (CsA), an immunosuppressive agent that targets the nuclear factor pathway of activated T-cells (NF-AT) to bind cellular cyclophilins (CyP), dose-dependently inhibited FIPV replication in vitro. FK506 (an immunosuppressor of the pathway that binds cellular FK506-binding protein (FKBP) but not CyP) did not affect FIPV replication. Neither cell growth nor viability changed in the presence of either CsA or FK506, and these factors did not affect the NF-AT pathway in fcwf-4 cells. Therefore, CsA does not seem to exert inhibitory effects via the NF-AT pathway. In conclusion, CsA inhibited FIPV replication in vitro and further studies are needed to verify the practical value of CsA as an anti-FIPV treatment in vivo.
猫传染性腹膜炎病毒（FIPV）是冠状病毒科的一员，可引起猫传染性腹膜炎（FIP），该病在猫中无法治愈且致命。环孢素 A（CsA）是一种免疫抑制剂，可靶向激活 T 细胞的核因子途径（NF-AT）以结合细胞亲环素（CyP），可剂量依赖性地抑制体外 FIPV 的复制。FK506（与细胞 FK506 结合蛋白（FKBP）结合而不是 CyP 的途径的免疫抑制剂）不影响 FIPV 的复制。CsA 或 FK506 的存在均不影响细胞生长或活力，并且这些因素也不影响 fcwf-4 细胞中的 NF-AT 途径。因此，CsA 似乎并非通过 NF-AT 途径发挥抑制作用。总之，CsA 抑制了体外 FIPV 的复制，需要进一步的研究来验证 CsA 作为体内抗 FIPV 治疗的实际价值。
