Dewerchin H L, Cornelissen E, Nauwynck H J
Laboratory of Virology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Arch Virol. 2005 Dec;150(12):2483-500. doi: 10.1007/s00705-005-0598-6. Epub 2005 Aug 1.
Feline infectious peritonitis virus (FIPV) (Coronaviridae) causes the most lethal viral infection in cats: FIP. The related feline enteric coronavirus (FECV) causes mild enteritis. Why these feline coronaviruses manifest so differently in vivo is not known. In this study, infection kinetics (titres and antigen expression) of FIPV 79-1146, and FECV 79-1683, were determined in peripheral blood monocytes from 3 donor cats and compared to those in Crandell feline kidney (CrFK) cells. The infection kinetics in monocytes were host dependent. Monocytes from 1 cat were resistant to both FIPV- and FECV-infection. Monocytes from the other 2 cats could initially be infected by both FIPV and FECV but FIPV infection was sustained in monocytes of only one cat. FECV-infection was never sustained and viral production was up to 100 times lower than in FIPV-infected monocytes. In CrFK cells, FIPV and FECV infection kinetics did not differ. In monocytes of a larger cat population (n = 19) the 3 infection patterns were also found. Considering all 22 investigated cats, 3/22 were not susceptible for FIPV and FECV. The rest could be infected with FECV and FIPV but 10/22 cats had monocytes that only sustained FIPV infection and 9/22 sustained neither FIPV nor FECV infection.
猫传染性腹膜炎病毒（FIPV）（冠状病毒科）可引发猫类最致命的病毒感染：猫传染性腹膜炎（FIP）。相关的猫肠道冠状病毒（FECV）会导致轻度肠炎。目前尚不清楚这些猫冠状病毒在体内表现为何如此不同。在本研究中，测定了来自3只供体猫的外周血单核细胞中FIPV 79 - 1146和FECV 79 - 1683的感染动力学（滴度和抗原表达），并与克兰德尔猫肾（CrFK）细胞中的情况进行了比较。单核细胞中的感染动力学取决于宿主。来自1只猫的单核细胞对FIPV和FECV感染均具有抗性。来自另外2只猫的单核细胞最初可被FIPV和FECV感染，但仅在其中1只猫的单核细胞中FIPV感染得以持续。FECV感染从未持续，其病毒产生量比FIPV感染的单核细胞低多达100倍。在CrFK细胞中，FIPV和FECV的感染动力学没有差异。在更大规模的猫群体（n = 19）的单核细胞中也发现了这3种感染模式。在所有22只被研究的猫中，22只中有3只对FIPV和FECV不敏感。其余的猫可被FECV和FIPV感染，但22只中有10只猫的单核细胞仅能持续FIPV感染，22只中有9只猫的单核细胞既不能持续FIPV感染也不能持续FECV感染。
