Yotsumoto S, Tsuyama S, Tashiro M, Murata F
Department of Dermatology, Faculty of Medicine, Kagoshima University, Japan.
J Histochem Cytochem. 1990 Dec;38(12):1815-21. doi: 10.1177/38.12.2254646.
We investigated the ultrastructure of blood group antigens A, B, and H in human eccrine glands by means of the immunogold labeling technique. Blood group antigens A, B, and H were found in the Golgi apparatus, secretory granules, and over the apical and basolateral cell membranes of dark cells of eccrine glands depending on the blood group phenotype of the donors. Both A and B antigens were found in the dark cells of AB donors. The labeling pattern of the Golgi stacks seemed to have a polarity whereby the anti-blood group A antibody labeled all the stacks, whereas anti-blood groups B and H bound to the trans side of the Golgi complex. These observations suggest that the blood group substances are secreted into the lumen after being processed through the Golgi apparatus and the immature and mature granules in the dark cells of human eccrine glands.
我们通过免疫金标记技术研究了人汗腺中血型抗原A、B和H的超微结构。根据供体的血型表型，在汗腺暗细胞的高尔基体、分泌颗粒以及顶端和基底外侧细胞膜上发现了血型抗原A、B和H。在AB型供体的暗细胞中同时发现了A和B抗原。高尔基体堆栈的标记模式似乎具有极性，即抗血型A抗体标记所有堆栈，而抗血型B和H抗体则与高尔基体复合体的反面结合。这些观察结果表明，血型物质在通过人汗腺暗细胞中的高尔基体以及未成熟和成熟颗粒进行加工后分泌到管腔中。
