Hanly J G, Pledger D
Department of Medicine, Victoria General Hospital, Nova Scotia, Canada.
Ann Rheum Dis. 1992 Sep;51(9):1066-8. doi: 10.1136/ard.51.9.1066.
The phenotypic characterization of enzymatically dissociated mononuclear cells in synovial membrane samples from multiple sites in two patients with rheumatoid arthritis (RA) were examined by fluorescence activated flow cytometry. In synovial membrane samples from each patient there was a consistent increase in the proportion of CD8+ cells (suppressor/cytotoxic), CD14+ cells (monocytes/macrophages), and HLA-DR+ cells compared with paired peripheral blood mononuclear cells. The proportion of CD4+ cells (helper/inducer) in synovial membrane was variable. Studies of in vitro production of IgM and IgM rheumatoid factor in one patient showed strikingly similar values for synovial membrane rheumatoid factor production at the two sites, which was enhanced compared with production in peripheral blood. These results suggest that in individual patients with RA the intra-articular immune response is comparable at multiple anatomical sites and that it is distinct from that in peripheral blood.
通过荧光激活流式细胞术对两名类风湿性关节炎（RA）患者多个部位滑膜膜样本中酶解单核细胞的表型特征进行了检测。与配对的外周血单核细胞相比，每名患者滑膜膜样本中CD8 +细胞（抑制/细胞毒性）、CD14 +细胞（单核细胞/巨噬细胞）和HLA-DR +细胞的比例持续增加。滑膜膜中CD4 +细胞（辅助/诱导）的比例各不相同。对一名患者体外产生IgM和IgM类风湿因子的研究表明，两个部位滑膜膜类风湿因子的产生值极为相似，与外周血中的产生相比有所增强。这些结果表明，在个体RA患者中，关节内免疫反应在多个解剖部位具有可比性，且与外周血中的免疫反应不同。
