European Centre of Environment and Human Health, Peninsula College of Medicine and Dentistry, University of Exeter , Truro, Cornwall, TR1 3HD , UK.
Nanotoxicology. 2013 Aug;7(5):963-73. doi: 10.3109/17435390.2012.689880. Epub 2012 Jul 27.
Surfactant protein D (SP-D) is primarily expressed in the lungs and modulates pro- and anti-inflammatory processes to toxic challenge, maintaining lung homeostasis. We investigated the interaction between NPs and SP-D and subsequent uptake by cells involved in lung immunity. Dynamic light scattering (DLS) and scanning electron microscopy (SEM) measured NP aggregation, particle size and charge in native human SP-D (NhSP-D) and recombinant fragment SP-D (rfhSP-D). SP-D aggregated NPs, especially following the addition of calcium. Immunohistochemical analysis of A549 epithelial cells investigated the co-localization of NPs and rfhSP-D. rfhSP-D enhanced the co-localisation of NPs to epithelial A549 cells in vitro. NP uptake by alveolar macrophages (AMs) and lung dendritic cells (LDCs) from C57BL/6 and SP-D knock-out mice were compared. AMs and LDCs showed decreased uptake of NPs in SP-D deficient mice compared to wild-type mice. These data confirmed an interaction between SP-D and NPs, and subsequent enhanced NP uptake.
表面活性蛋白 D（SP-D）主要在肺部表达，调节对有毒物质的促炎和抗炎过程，维持肺部内环境稳定。我们研究了 NP 与 SP-D 之间的相互作用，以及随后这些 NP 被涉及肺部免疫的细胞摄取的情况。动态光散射（DLS）和扫描电子显微镜（SEM）测量了天然人 SP-D（NhSP-D）和重组片段 SP-D（rfhSP-D）中 NP 的聚集、粒径和电荷。SP-D 聚集了 NP，尤其是在添加钙后。用免疫组化分析 A549 上皮细胞研究了 NP 和 rfhSP-D 的共定位。体外实验中，rfhSP-D 增强了 NP 与上皮 A549 细胞的共定位。比较了 C57BL/6 和 SP-D 基因敲除小鼠的肺泡巨噬细胞（AMs）和肺树突状细胞（LDCs）对 NP 的摄取。与野生型小鼠相比，SP-D 缺失小鼠的 AMs 和 LDCs 对 NP 的摄取减少。这些数据证实了 SP-D 与 NP 之间的相互作用，以及随后增强的 NP 摄取。
