Aerosol and Air Quality Research Laboratory, Department of Energy, Environmental & Chemical Engineering, Washington University in St. Louis, St. Louis, MO 63130, USA.
J Aerosol Med Pulm Drug Deliv. 2012 Dec;25(6):355-64. doi: 10.1089/jamp.2011.0967. Epub 2012 May 2.
Air-jet atomization is a common technique used for the generation of therapeutic aerosols from liposome suspensions for drug delivery to the lungs. Although the technique does not use an electric field, the aerosols generated by this technique are still charged, and this may affect respiratory drug deposition.
In this study, the charge distribution of liposomes aerosolized by an air-jet atomizer was measured using a tandem differential mobility analyzer (TDMA) technique. The liposomes, composed of a mixture of two amphiphilic lipids and cholesterol, were synthesized by the dehydration-rehydration vesicle method. The effect of the precursor suspension properties, such as medium composition, pH, conductivity, and lipid mass concentration, on the charge distribution of the liposome aerosols was studied.
Results showed that the atomized liposomes have a bipolar charge distribution, and the number-fraction of charged liposome aerosols was influenced strongly by properties of the precursor solution under investigation. Liposomes synthesized in deionized water were observed to carry much higher charges than those synthesized in phosphate-buffered saline (PBS). Increasing the lipid mass concentration in the precursor suspension resulted in a decrease in the charge on the aerosols. Thus, the precursor suspension properties--composition, pH, and conductivity--can be used to control the magnitude of charge on liposome aerosols and to synthesize engineered liposome particles for the pulmonary delivery of drugs with controlled alveolar deposition and controlled delivery to alveolar macrophages.
空气喷射雾化是一种常用的技术，用于从脂质体悬浮液中生成治疗性气溶胶，以将药物递送到肺部。尽管该技术不使用电场，但通过该技术产生的气溶胶仍然带有电荷，这可能会影响呼吸道药物沉积。
在这项研究中，使用串联差分迁移率分析仪（TDMA）技术测量了空气喷射雾化器雾化的脂质体的电荷分布。脂质体由两种两亲脂质和胆固醇的混合物组成，通过脱水再水化囊泡法合成。研究了前体悬浮液性质（如介质组成、pH 值、电导率和脂质质量浓度）对脂质体气溶胶电荷分布的影响。
结果表明，雾化的脂质体具有双极电荷分布，所研究的前体溶液的性质强烈影响带电荷的脂质体气溶胶的数量分数。在去离子水中合成的脂质体比在磷酸盐缓冲盐水（PBS）中合成的脂质体携带更高的电荷。在前体悬浮液中增加脂质质量浓度会导致气溶胶上的电荷减少。因此，可以使用前体悬浮液的性质（组成、pH 值和电导率）来控制脂质体气溶胶的电荷量，并合成用于肺部递药的工程化脂质体颗粒，以控制肺泡沉积和对肺泡巨噬细胞的控制释放。
