Institute of Food Chemistry, Westfälische Wilhelms-Universität Münster, Münster, Germany.
J Agric Food Chem. 2012 Jun 6;60(22):5676-84. doi: 10.1021/jf300634k. Epub 2012 May 24.
The mycotoxin T-2 toxin, produced by various Fusarium species, is a widespread contaminant of grain and grain products. Knowledge about its toxicity and metabolism in the human body is crucial for any risk assessment as T-2 toxin can be detected in processed and unprocessed food samples. Cell culture studies using cells of human origin represent a potent model system to study the metabolic fate of T-2 toxin as well as the cytotoxicity in vitro. In this study the metabolism of T-2 toxin was analyzed in a cell line derived from human colon carcinoma cells (HT-29) and primary human renal proximal tubule epithelial cells (RPTEC) using high-performance liquid chromatography coupled with Fourier transformation mass spectrometry (HPLC-FTMS). Both cell types metabolized T-2 toxin to a variety of compounds. Furthermore, cell cycle analysis in RPTEC proved the apoptotic effect of T-2 toxin and its metabolites HT-2 toxin and neosolaniol in micromolar concentrations.
真菌镰孢属产生的霉菌毒素 T-2 毒素是谷物及其制品中广泛存在的污染物。由于 T-2 毒素可在加工和未加工的食品样本中被检测到，因此了解其在人体内的毒性和代谢情况对于任何风险评估都至关重要。用人源细胞进行的细胞培养研究代表了一种强大的模型系统，可用于研究 T-2 毒素的代谢命运以及体外细胞毒性。在这项研究中，使用高效液相色谱法与傅里叶变换质谱法（HPLC-FTMS）分析了源自人结肠癌细胞（HT-29）和原代人肾近端小管上皮细胞（RPTEC）的细胞系中 T-2 毒素的代谢情况。这两种细胞类型都将 T-2 毒素代谢为多种化合物。此外，RPTEC 的细胞周期分析证明了 T-2 毒素及其代谢物 HT-2 毒素和 neo 索兰醇在微摩尔浓度下的凋亡作用。
