University of Münster, Wilhelm-Klemm-Strasse 10, D-48149 Münster, Germany.
J Agric Food Chem. 2011 Jul 27;59(14):7798-807. doi: 10.1021/jf2012024. Epub 2011 Jun 23.
Ergot alkaloids are known toxic secondary metabolites of the fungus Claviceps purpurea occurring in various grains, especially rye products. The liver is responsible for converting the ergot alkaloids into metabolites; however, the toxic impact of these end products of metabolism is still unknown. The aim of this study was to analyze the metabolism of ergot alkaloids in colon and liver cell lines (HT-29, HepG2), as well as in human primary renal cells (RPTEC). It was shown that cells in vitro are able to metabolize ergot alkaloids, forming a variety of metabolic compounds. Significant differences between the used cell types could be identified, and a suitable model system was established using HT-29 cells, performing an intensive metabolism to hydroxylated metabolites. The formed substances were analyzed by coupling of high-performance liquid chromatography with fluorescence detection and Fourier transformation mass spectrometry (HPLC-FLD-FTMS) as a powerful tool to identify known and unknown metabolites.
麦角生物碱是真菌麦角菌产生的已知有毒次生代谢物，存在于各种谷物中，尤其是黑麦产品。肝脏负责将麦角生物碱转化为代谢物；然而，这些代谢终产物的毒性影响仍不清楚。本研究旨在分析麦角生物碱在结肠和肝细胞系（HT-29、HepG2）以及人原代肾细胞（RPTEC）中的代谢情况。结果表明，体外细胞能够代谢麦角生物碱，形成多种代谢化合物。可以鉴定出所使用的细胞类型之间存在显著差异，并使用 HT-29 细胞建立了合适的模型系统，对羟基化代谢物进行了强化代谢。通过高效液相色谱法与荧光检测和傅里叶变换质谱法（HPLC-FLD-FTMS）相结合来分析形成的物质，作为一种识别已知和未知代谢物的强大工具。
