Department of Animal Sciences, University of Ljubljana, Groblje 3, SI-1230 Domzale, Slovenia.
J Hazard Mater. 2012 Jun 30;221-222:199-205. doi: 10.1016/j.jhazmat.2012.04.029. Epub 2012 Apr 20.
We provide experimental evidence that changes in the membrane fatty acid profile of Tetrahymena thermophila incubated with nano- or bulk TiO(2) particle are not accompanied by ROS generation or lipid peroxidation. Consequently these changes are interpreted as acclimation to unfavorable conditions and not as toxic effects. T. thermophila cells were exposed to TiO(2) particles at different concentrations for 24h at 32°C. Treatment of cultures with nano- and bulk TiO(2) particles resulted in changes of membrane fatty acid profile, indicating increased membrane rigidity, but no lipid peroxidation or ROS generation was detected. There were no differences in membrane composition when T. thermophila was exposed to nanosized or bulk-TiO(2) particles. We also observed reversible filling of food vacuoles, but this was different in case of nano- or bulk TiO(2) exposure. Our results suggest that interactions of particles and cell membranes are independent of oxidative stress.
我们提供的实验证据表明，与纳米或块状 TiO(2) 颗粒孵育的嗜热四膜虫的膜脂肪酸谱的变化并不伴有 ROS 生成或脂质过氧化。因此，这些变化被解释为对不利条件的适应，而不是毒性作用。嗜热四膜虫细胞在 32°C 下用不同浓度的 TiO(2) 颗粒暴露 24 小时。用纳米和块状 TiO(2) 颗粒处理培养物导致膜脂肪酸谱发生变化，表明膜刚性增加，但未检测到脂质过氧化或 ROS 生成。当嗜热四膜虫暴露于纳米或块状 TiO(2) 颗粒时，膜组成没有差异。我们还观察到食物泡的可逆填充，但纳米或块状 TiO(2) 暴露的情况不同。我们的结果表明，颗粒与细胞膜的相互作用不依赖于氧化应激。
