Department of Rehabilitation Medicine, Jiangsu Lake Taihu Cadres Sanatorium of Jiangsu Provincial People's Hospital Group, Wuxi, Jiangsu 214086, PR China.
Mol Med Rep. 2012 Jul;6(1):69-74. doi: 10.3892/mmr.2012.897. Epub 2012 May 2.
The aim of the present study was to explore the role of exercise in diabetic peripheral neuropathy (DPN) and the mechanisms involved. For this purpose, 31 male Sprague-Dawley rats were used. The rats were assigned to 5 groups: diabetic rats subjected to exercise training (swimming) for 8 weeks (D-Ex1 group), diabetic rats subjected to exercise training for 4 weeks after 4 weeks of being sedentary (D-Ex2 group), diabetic rats which remained sedentary for 8 weeks (D-Sed group), control rats subjected to exercise training for 8 weeks (C-Ex1 group) and control rats which remained sedentary for 8 weeks (C-Sed group). Blood glucose levels and caudal nerve conduction velocity (NCV) were evaluated at 0 (baseline), 28 (4 weeks) and 56 days (8 weeks) after the induction of diabetes. The levels of neurotrophin-3 (NT-3) in skeletal muscle were measured by ELISA at the end of the experiment. Blood glucose levels in the D-Ex1 group rats decreased significantly after 8 weeks of exercise. The caudal NCV markedly decreased in all diabetic rats and significantly increased after 4 or 8 weeks of exercise training. Muscle NT-3 levels were significantly lower in the D-Sed compared to the 4 other groups. Muscle NT-3 levels positively correlated with caudal NCV. In conclusion, swimming training has a beneficial effect on DPN and muscle NT-3 levels, which could help improve caudal NCV in streptozotocin-induced diabetic rats.
本研究旨在探讨运动在糖尿病周围神经病变（DPN）中的作用及其相关机制。为此，使用了 31 只雄性 Sprague-Dawley 大鼠。将大鼠分为 5 组：8 周游泳运动训练的糖尿病大鼠（D-Ex1 组）、4 周静息后 4 周运动训练的糖尿病大鼠（D-Ex2 组）、8 周静息的糖尿病大鼠（D-Sed 组）、8 周运动训练的正常大鼠（C-Ex1 组）和 8 周静息的正常大鼠（C-Sed 组）。在诱导糖尿病后 0（基线）、28（4 周）和 56 天（8 周）时评估血糖水平和尾神经传导速度（NCV）。实验结束时通过 ELISA 测定骨骼肌中神经营养因子-3（NT-3）的水平。D-Ex1 组大鼠经过 8 周的运动后血糖水平显著降低。所有糖尿病大鼠的尾 NCV 明显降低，经过 4 或 8 周的运动训练后显著增加。与其他 4 组相比，D-Sed 组的肌肉 NT-3 水平明显降低。肌肉 NT-3 水平与尾 NCV 呈正相关。结论：游泳训练对 DPN 和肌肉 NT-3 水平有有益影响，可能有助于改善链脲佐菌素诱导的糖尿病大鼠的尾 NCV。
