Department of Physiology, Jawaharlal Institute of Post-graduate Medical Education and Research (JIPMER), Puducherry, India.
Department of Biochemistry, Jawaharlal Institute of Post-graduate Medical Education and Research (JIPMER), Puducherry, India.
PLoS One. 2021 Jan 15;16(1):e0245395. doi: 10.1371/journal.pone.0245395. eCollection 2021.
Diabetic neuropathy is the most common complication affecting more than 50% of patients with longstanding diabetes. Till date, there are no reports to explain the scientific basis of alternative medicine as an adjunct therapy for treating diabetic neuropathy. Hence, we studied the effect of honey and insulin treatment on hyperglycemia, dyslipidemia, oxidant and anti-oxidant status and nerve conduction in experimental diabetic neuropathy Wistar rats. In this experimental study, forty healthy male Wistar albino rats of 10-12 weeks age, weighing between 150 to 200g were obtained from our institute central animal house. After acclimatization, the rats were divided into control (n = 8) and experimental (n = 32) groups randomly. In the experimental group, type 2 diabetic neuropathy was induced with high fat and high sugar diet for 8 weeks followed by streptozotocin at a dose of 35 mg/kg body weight. Three days after streptozotocin injection, blood glucose levels of rats were measured from fasting samples to confirm diabetes. After the development of diabetes, rats were given standard rodent chow and allowed four more weeks to remain diabetic and to develop neuropathy. Every second week, nerve conduction study was done to confirm neuropathy. All the diabetic rats of experimental group developed neuropathy after 4 weeks of developing diabetes, which was confirmed by significant reduction in conduction velocity of sensory and motor nerve when compared to non-diabetic control group. After the development of neuropathy, these rats were randomly divided into diabetic neuropathy with no treatment group (n = 8) and three treatment groups (n = 8, each). The rats of treatment group were administered with either honey or insulin or honey+insulin for six weeks. After six-weeks of intervention, there was significant decrease in blood glucose and lipids in honey, insulin and honey+insulin treated neuropathy rats, when compared with no treatment group. Malondialdehyde was reduced and total anti-oxidant status improved in all the three treatment groups. There was no significant increase in conduction velocity of sciatic tibial motor nerve in treatment groups when compared with no treatment group. However, the sensory nerve conduction velocity improved significantly in honey+insulin treated neuropathy rats. In conclusion, six-week honey treatment helped in reducing dyslipidemia and oxidative stress. Honey given along with insulin for six-weeks improved sensory nerve conduction velocity in experimental diabetic neuropathy Wistar rats.
糖尿病周围神经病变是最常见的并发症，影响超过 50%的长期糖尿病患者。迄今为止，尚无报告解释替代医学作为治疗糖尿病周围神经病变的辅助疗法的科学依据。因此，我们研究了蜂蜜和胰岛素治疗对实验性糖尿病周围神经病变 Wistar 大鼠高血糖、血脂异常、氧化应激和抗氧化状态及神经传导的影响。在这项实验研究中，我们从研究所中心动物房获得了 40 只 10-12 周龄、体重在 150 至 200 克之间的健康雄性 Wistar 白化大鼠。适应环境后，将大鼠随机分为对照组（n = 8）和实验组（n = 32）。在实验组中，大鼠用高脂肪和高糖饮食喂养 8 周，然后用 35mg/kg 体重的链脲佐菌素诱导 2 型糖尿病周围神经病变。链脲佐菌素注射后 3 天，从空腹样本中测量大鼠的血糖水平以确认糖尿病。糖尿病发生后，给予大鼠标准啮齿动物饲料，并允许再持续 4 周保持糖尿病状态并发展为神经病变。每两周进行一次神经传导研究，以确认神经病变。实验组的所有糖尿病大鼠在发生糖尿病 4 周后均发展为神经病变，与非糖尿病对照组相比，感觉和运动神经的传导速度显著降低，这一点得到了证实。神经病变发生后，这些大鼠被随机分为无治疗组（n = 8）和三组治疗组（n = 8，每组）。治疗组大鼠分别给予蜂蜜、胰岛素或蜂蜜+胰岛素治疗 6 周。干预 6 周后，与未治疗组相比，蜂蜜、胰岛素和蜂蜜+胰岛素治疗神经病变大鼠的血糖和血脂明显下降。丙二醛减少，所有三组的总抗氧化状态均得到改善。与未治疗组相比，治疗组坐骨神经胫后运动神经的传导速度没有显著增加。然而，蜂蜜+胰岛素治疗神经病变大鼠的感觉神经传导速度显著改善。结论：6 周的蜂蜜治疗有助于降低血脂异常和氧化应激。6 周的蜂蜜联合胰岛素治疗可改善实验性糖尿病周围神经病变 Wistar 大鼠的感觉神经传导速度。
