Shanghai Institute of Biological Products, Shanghai 200052, China.
Arch Virol. 2012 Aug;157(8):1451-61. doi: 10.1007/s00705-012-1318-7. Epub 2012 May 4.
Our previous studies have proven the adjuvanticity of chitosan in mice when administered with inactivated and subunit influenza vaccine. In this study, we investigated the adjuvant effect of chitosan on the immunogenicity and protective efficacy of a live attenuated influenza vaccine. Mice were inoculated intranasally with live attenuated influenza vaccine plus chitosan and then challenged with a high, lethal dose of homologous or heterologous virus. Antibody responses, secretion of IFN-γ by spleen cells, body weight loss, survival rates, and residual lung virus titers were tested. The results demonstrated that live attenuated influenza vaccine with chitosan adjuvant not only protected mice completely against challenge with the homologous virus but also provided good cross-protection against a heterologous virus. In addition, chitosan as adjuvant could significantly increase the levels of antigen-specific antibodies and the population of IFN-γ-secreting T cells. These results reveal the potential of chitosan as a candidate adjuvant for use in a live attenuated influenza vaccine.
我们之前的研究已经证明了壳聚糖在给小鼠接种灭活和亚单位流感疫苗时的佐剂作用。在这项研究中，我们研究了壳聚糖对减毒活流感疫苗的免疫原性和保护效力的佐剂作用。通过鼻腔接种将减毒活流感疫苗与壳聚糖一起接种给小鼠，然后用同源或异源高致死剂量的病毒进行攻毒。检测了抗体反应、脾细胞分泌 IFN-γ、体重减轻、存活率和肺内残留病毒滴度。结果表明，用壳聚糖佐剂的减毒活流感疫苗不仅能完全保护小鼠免受同源病毒的攻击，而且还能对异源病毒提供良好的交叉保护。此外，壳聚糖作为佐剂可以显著增加抗原特异性抗体的水平和 IFN-γ 分泌 T 细胞的数量。这些结果揭示了壳聚糖作为减毒活流感疫苗候选佐剂的潜力。
