Department of Respiratory Medicine, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210011, PR China.
Mol Med Rep. 2012 Jul;6(1):100-4. doi: 10.3892/mmr.2012.893. Epub 2012 Apr 26.
Chrysin, a flavonoid obtained from various natural sources, has been reported to possess anti-inflammatory, antitumor, antioxidant and anti-allergic activities. However, its anti-inflammatory and immunoregulatory activities in asthma animal models are poorly understood. In the present study, we examined the effects of chrysin on airway inflammation and the possible mechanisms through which it acts in a murine model of allergic asthma. BALB/c mice sensitized and challenged to ovalbumin (OVA) were administered intragastrically with chrysin at a dose of 50 mg/kg daily. Chrysin significantly suppressed OVA-induced airway hyperresponsiveness (AHR) to acetylcholine chloride (Ach). Chrysin administration significantly inhibited the total inflammatory cell and eosinophil counts in bronchoalveolar lavage fluid (BALF) and total immunoglobulin E (IgE) levels in serum. Histological examination of lung tissue demonstrated that chrysin significantly attenuated allergen-induced lung eosinophilic inflammation and mucus-producing goblet cells in the airway. In addition, chrysin triggered a switch of the immune response to allergens towards a T-helper type 1 (Th1) profile by modulating the transcription factors T-bet and GATA-3 in allergic mice. These data suggest that chrysin exhibits anti-inflammatory and immunoregulatory properties and provides new insights into the immunopharmacological role of chrysin in terms of its effects in a murine model of asthma.
白杨素是一种从各种天然来源中获得的黄酮类化合物，据报道具有抗炎、抗肿瘤、抗氧化和抗过敏活性。然而，其在哮喘动物模型中的抗炎和免疫调节活性知之甚少。在本研究中，我们研究了白杨素对气道炎症的影响及其在变应性哮喘小鼠模型中作用的可能机制。卵清蛋白（OVA）致敏和激发的 BALB/c 小鼠每日给予白杨素 50mg/kg 灌胃。白杨素显著抑制 OVA 诱导的乙酰胆碱氯（Ach）气道高反应性（AHR）。白杨素给药显著抑制了支气管肺泡灌洗液（BALF）中总炎性细胞和嗜酸性粒细胞计数以及血清中总免疫球蛋白 E（IgE）水平。肺组织的组织学检查表明，白杨素显著减轻了过敏原诱导的肺嗜酸性粒细胞炎症和气道中产生粘液的杯状细胞。此外，白杨素通过调节变应原小鼠中的转录因子 T-bet 和 GATA-3，引发了针对过敏原的免疫反应向 T 辅助型 1（Th1）表型的转变。这些数据表明，白杨素具有抗炎和免疫调节特性，并为白杨素在哮喘小鼠模型中的免疫药理学作用提供了新的见解。
