含醉鱼草黄酮类药物血清对体外培养泪腺上皮细胞STAT1磷酸化蛋白表达的影响

Effect of Buddleia flavonoids drug-containing plasma on the expression of STAT1 phosphoprotein in lacrimal gland epithelial cells in vitro.

作者信息

Wang Fang, Peng Qing-Hua, Yao Xiao-Lei, Wu Quan-Long, Li Dian

机构信息

Department of Ophthalmology, Second Affiliated Hospital of Guiyang University of Traditional Chinese Medicine, Guiyang 550003, Guizhou Province, China.

出版信息

Int J Ophthalmol. 2010;3(1):32-5. doi: 10.3980/j.issn.2222-3959.2010.01.08. Epub 2010 Mar 18.

DOI:10.3980/j.issn.2222-3959.2010.01.08

PMID:22553513

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3340662/

Abstract

AIM

To explore the effect of Buddleia flavonoids drug-containing plasma and androgen receptor (AR) blocker on the expression of STAT1 phosphoprotein.

METHODS

In vitro lacrimal gland epithelial cells were cultivated with H(2)O(2) to establish the dry eye apoptosis state. Blank plasma group, Buddleia officinalis plasma total flavonoids interfere with drug-containing group, and the intervention group of testosterone propionate were set. The expressions of STAT1 phosphoprotein of each group were observed by Western blot. AR blocker flutamide was used to explore the intended androgen effect of Buddleia flavonoids.

RESULTS

After the intervention of drug-containing plasma, the expression of STAT1 phosphoprotein in Buddleja officinalis drug-containing plasma intervention group (0.353±0.494) and testosterone propionate intervention group (0.502±0.036) were enhanced and the differences between the two groups were significant (P<0.01). After using AR blocker, the expression of STAT1 phosphoprotein in each group (0.268±0.061, 0.283±0.106, 0.213±0.071) had no difference.

CONCLUSION

Buddleja officinalis drug-containing plasma total flavonoids can promote the expression of STAT1 phosphorylation.

摘要

目的

探讨密蒙花黄酮含药血清及雄激素受体（AR）阻断剂对信号转导和转录激活因子1（STAT1）磷酸化蛋白表达的影响。

方法

体外培养泪腺上皮细胞，用H₂O₂诱导建立干眼细胞凋亡状态。设空白血清组、密蒙花总黄酮含药血清干预组、丙酸睾酮干预组，采用蛋白质免疫印迹法（Western blot）观察各组STAT1磷酸化蛋白表达情况，并用AR阻断剂氟他胺探讨密蒙花黄酮的拟雄激素效应。

结果

含药血清干预后，密蒙花含药血清干预组（0.353±0.494）及丙酸睾酮干预组（0.502±0.036）的STAT1磷酸化蛋白表达增强，两组比较差异有统计学意义（P<0.01）。应用AR阻断剂后，各组STAT1磷酸化蛋白表达（0.268±0.061，0.283±0.106，0.213±0.071）差异无统计学意义。