密蒙花提取物对去势干眼兔泪腺局部炎症的影响

Effects of extract of Buddleja officinalis on partial inflammation of lacrimal gland in castrated rabbits with dry eye.

作者信息

Yao Xiao-Lei, Peng Qing-Hua, Peng Jun, Tan Han-Yu, Wu Quan-Long, Wu Da-Li, Chen Mei, Li Chuan-Ke, Li Dian, Zhu Hui-An

机构信息

Department of Ophthalmology, the First Affiliated Hospital of Hunan University of Traditional Chinese Medicine, Changsha 410007, Hunan Province, China.

出版信息

Int J Ophthalmol. 2010;3(2):114-9. doi: 10.3980/j.issn.2222-3959.2010.02.05. Epub 2010 Jun 18.

DOI:10.3980/j.issn.2222-3959.2010.02.05

PMID:22553532

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3340767/

Abstract

AIM

To assess the effects of extract of Buddleja officinalis on tear secretion volume, tear film stability, expressions of TGF-β1, IL-1β, TNF-α in lacrimal gland of castrated rabbits with dry eye.

METHODS

A total of 30 victory rabbits were divided averagely into normal group(A), model group(B), therapy group with low dose extract of Buddleja officinalis (C), therapy group with high dose extract of Buddleja officinalis (D) and therapy group with genistein (E). The dry eye model was established with orchiectomy on Group B, C, D, E. Group C, D, E were administered intragastrically with corresponding dose extract of Buddleja officinalis or genistein for 30 days. All rabbits were detected with SIT. TGF-β1, IL-1β, TNF-α were detected with immunohistochemistry and the ultrastructure of lacrimal gland was observed under transmission electron microscope.

RESULTS

The SIT value of group C, D, E were respectively 13.167±4.957, 14.667±5.279, 8.667±0.516, obviously higher than that of group B 5.667±2.338 (P<0.01). The positive expression of IL-1β in acinar cell and glandular tube cell of group C, D were 0.470±0.048, 0.510±0.088, obviously lower than that of group B 0.770±0.118 (P<0.01). The positive expression of TNF-α of group C, D were 0.498±0.156, 0.435±0.069, obviously lower than that of group B 0.769±0.095 too (P<0.01). The positive expression of TGF-β1 of group C, D were 0.406±0.171, 0.497±0.147, obviously higher than that of group B 0.222±0.113(P<0.01). Any result of group C, D was positive compared with that of group E (P <0.05). Ultrastructure of the lacrimal gland of group C, D, E was well preserved, especially in D group it was remarkable.

CONCLUSION

The extract of Buddleja officinalis can adjust lacrimal gland partial inflammation of dry eye.

摘要

目的

评估密蒙花提取物对去势干眼兔泪液分泌量、泪膜稳定性及泪腺中转化生长因子-β1（TGF-β1）、白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）表达的影响。

方法

将30只成年雄性兔平均分为正常组（A组）、模型组（B组）、密蒙花提取物低剂量治疗组（C组）、密蒙花提取物高剂量治疗组（D组）和金雀异黄素治疗组（E组）。对B、C、D、E组行睾丸摘除术建立干眼模型。C、D、E组分别灌胃相应剂量的密蒙花提取物或金雀异黄素，连续30天。所有兔均行SchirmerⅠ试验（SIT）检测。采用免疫组织化学法检测TGF-β1、IL-1β、TNF-α，透射电子显微镜下观察泪腺超微结构。

结果

C、D、E组SIT值分别为13.167±4.957、14.667±5.279、8.667±0.516，明显高于B组的5.667±2.338（P<0.01）。C、D组腺泡细胞和腺管细胞中IL-1β阳性表达分别为0.470±0.048、0.510±0.088，明显低于B组的0.770±0.118（P<0.01）。C、D组TNF-α阳性表达分别为0.498±0.156、0.435±0.069，也明显低于B组的0.769±0.095（P<0.01）。C、D组TGF-β1阳性表达分别为0.406±0.171、0.497±0.147，明显高于B组的0.222±0.113（P<0.01）。C、D组各项结果与E组比较，差异均有统计学意义（P<0.05）。C、D、E组泪腺超微结构保存良好，尤以D组显著。