黄酮对眼部血流及脉络膜新生血管形成的影响。
Effect of flavone on the ocular blood flow and formation of choroidal neovascularization.
作者信息
Zhuang Pei, Shen Yi, Chiou George C Y
机构信息
Institute of Ocular Pharmacology, College of Medicine, Texas A&M Health Science Center, College Station, TX 77843, USA.
出版信息
Int J Ophthalmol. 2010;3(2):95-8. doi: 10.3980/j.issn.2222-3959.2010.02.01. Epub 2010 Jun 18.
AIM
To investigate the effect of flavone on ocular blood flow in rabbit eyes and the formation of choroidal neovascularization (CNV) in rat model of age-related macular degeneration (AMD).
METHODS
In in vivo studies, colored microsphere technique was used to determine the ocular blood flow in ocular hypertensive rabbit eyes. The rat eyes were treated with 0.5% flavone eye drops 3 times a day for 1 week before and 4 weeks after laser-induced injury of Bruch's membrane. The development of CNV was determined by fluorescein angiography (FA) performed on the 2nd and 4th after injury. In in vitro studies, the effect of flavone on the viability of human umbilical vein endothelial cells HUVECs was measured by MTT assay.
RESULTS
The ocular blood flow in rabbit eyes was significantly increased after flavone instillation. Flavone significantly inhibited the formation of laser induced CNV. In vitro results showed that flavone inhibited the proliferation of HUVECs.
CONCLUSION
Flavone could increase ocular blood flow and inhibit the formation of CNV.
目的
研究黄酮对兔眼眼部血流以及年龄相关性黄斑变性（AMD）大鼠模型脉络膜新生血管（CNV）形成的影响。
方法
在体内研究中，采用彩色微球技术测定高眼压兔眼的眼部血流。在激光诱导 Bruch 膜损伤前 1 周及损伤后 4 周，每天 3 次用 0.5%黄酮滴眼液处理大鼠眼睛。在损伤后第 2 天和第 4 天通过荧光素血管造影（FA）确定 CNV 的发展情况。在体外研究中，通过 MTT 法测定黄酮对人脐静脉内皮细胞（HUVECs）活力的影响。
结果
滴注黄酮后兔眼的眼部血流显著增加。黄酮显著抑制激光诱导的 CNV 形成。体外结果表明黄酮抑制 HUVECs 的增殖。
结论
黄酮可增加眼部血流并抑制 CNV 的形成。
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