Li Cai-Rui, Sun Shu-Guang
Department of Ophthalmology, Hospital of Dali University, Dali 671000, Yunnan Province, China.
Int J Ophthalmol. 2010;3(3):224-7. doi: 10.3980/j.issn.2222-3959.2010.03.10. Epub 2010 Sep 18.
To investigate retinal vascular endothelial growth factor (VEGF) level and retinal cells apoptosis in the early stage of diabetic NOD mouse retina.
Animals were divided into non-diabetes group, (control) (2-, 4-, 6-, 8- and 12-week sub-groups, n=30) and diabetes group (2-, 4-, 6-, 8- and 12-week sub-groups, n=30). Enzyme-linked immunosorbent assay (ELISA) was performed to detect VEGF level in both serum and retina. Transmission electron microscope method was used to examine retinal cell apoptosis.
Compared with the control group, VEGF levels in serum and retina were increased significantly in the NOD group (12 weeks: 4.9±0.4µg/g vs 0.19±0.1µg/g in serum sample, P<0.01; 165±9µg/g vs 17±5µg/g in retinal sample, P<0.01). There exists a positive correlation between serum VEGF and retinal VEGF levels in the early diabetic NOD mice (γ=0.9902, P=0.001). The number of the cells apoptosis in the ganglion cells and endothelium can also been found increased significantly in the NOD group (P<0.01).
The high VEGF expression may be contributed to increased retinal cells apoptosis. Many factors associated with retinal VEGF expression might involve in the early diabetes stage.
研究糖尿病NOD小鼠视网膜早期视网膜血管内皮生长因子（VEGF）水平及视网膜细胞凋亡情况。
将动物分为非糖尿病组（对照组）（2周、4周、6周、8周和12周亚组，n = 30）和糖尿病组（2周、4周、6周、8周和12周亚组，n = 30）。采用酶联免疫吸附测定（ELISA）法检测血清和视网膜中的VEGF水平。用透射电子显微镜法检测视网膜细胞凋亡情况。
与对照组相比，NOD组血清和视网膜中的VEGF水平显著升高（12周时：血清样本中为4.9±0.4μg/g，对照组为0.19±0.1μg/g，P<0.01；视网膜样本中为165±9μg/g，对照组为17±5μg/g，P<0.01）。糖尿病早期NOD小鼠血清VEGF与视网膜VEGF水平之间存在正相关（γ = 0.9902，P = 0.001）。NOD组神经节细胞和内皮细胞的凋亡数量也显著增加（P<0.01）。
VEGF高表达可能导致视网膜细胞凋亡增加。许多与视网膜VEGF表达相关的因素可能参与糖尿病早期阶段。
