榄香烯体外抑制人晶状体上皮细胞增殖的机制
Mechanisms of inhibition of elemene on human lens epithelial cell proliferation in vitro.
作者信息
Hu Yan-Hong, Huang Xiu-Rong, Qi Ming-Xin, Hou Bu-Yuan
机构信息
Department of Ophthalmology, Second Affiliated Peoples Hospital of Fujian College of Traditional Chinese Medicine, Fuzhou 350003, Fujian Province, China.
出版信息
Int J Ophthalmol. 2011;4(3):255-8. doi: 10.3980/j.issn.2222-3959.2011.03.08. Epub 2011 Jun 18.
AIM
To study the effects of elemene (Ele) on proliferation and cell cycle of human lens epithelial cells B3 (HLE-B3) and the mechanisms of its signal transduction.
METHODS
Recombinant human basic fibroblast growth factor (rhbFGF) was used to induce proliferation of HLE-B3 cells, which were incubated with 80mg/L Ele for 24 hours. The inhibitory effects of Ele on the proliferation of HLE-B3 cells were evaluated by MTT method. The effect of Ele on HLE-B3 cell cycle was analyzed by flow cytometry(FCM). The expressions of protein kinase A (PKA) and protein kinase G (PKG) of HLE-B3 were also analyzed by FCM.
RESULTS
Ele altered the cell cycle of HLE-B3 and effectively inhibited HLE-B3 cell proliferation induced by rhbFGF. Ele up-regulated PKA and down-regulated the expression of PKG in HLE-B3 cell.
CONCLUSION
Ele inhibits HLE-B3 proliferation, making it an attractive potential agent in regimens to treat after-cataracts.
目的
研究榄香烯（Ele）对人晶状体上皮细胞B3（HLE - B3）增殖及细胞周期的影响及其信号转导机制。
方法
采用重组人碱性成纤维细胞生长因子（rhbFGF）诱导HLE - B3细胞增殖，将其与80mg/L榄香烯孵育24小时。采用MTT法评估榄香烯对HLE - B3细胞增殖的抑制作用。通过流式细胞术（FCM）分析榄香烯对HLE - B3细胞周期的影响。同时采用FCM分析HLE - B3中蛋白激酶A（PKA）和蛋白激酶G（PKG）的表达。
结果
榄香烯改变了HLE - B3的细胞周期，并有效抑制了rhbFGF诱导的HLE - B3细胞增殖。榄香烯上调HLE - B3细胞中PKA的表达并下调PKG的表达。
结论
榄香烯抑制HLE - B3增殖，使其成为治疗后发性白内障方案中一种有吸引力的潜在药物。
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