Zhu Dan, Li Peng, Wang Li, He Yuan, Zhang Hui-Zi
Department of Optometry, Xi'an Medical University, Xi'an 710021, Shaanxi Province, China.
Department of Ophthalmology, Xijing 986 Hospital Department, Fourth Military Medical University, Xi'an 710054, Shaanxi Province, China.
Int J Ophthalmol. 2023 Mar 18;16(3):333-341. doi: 10.18240/ijo.2023.03.01. eCollection 2023.
To evaluate the regulation of the aberrant expression of () in the development of age-related cataract (ARC).
Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blot analysis were employed to evaluate the expression of in ARC patients and healthy controls. The proliferation, apoptosis, cell cycle and epithelial-mesenchymal transition (EMT) of human lens epithelial cell (HLE-B3) were further analyzed under the condition of gene silence. Alteration of gene expression at mRNA level after knockdown were also evaluated by qRT-PCR on HLE-B3 cells.
The aberrant expression of was identified a clinically associated with the ARC. Silencing of promoted the apoptosis and inhibited the proliferation of HLE-B3 by blocking the cell cycle. Moreover, gene silence didn't affect the cytoskeleton of HLE-B3 but down-regulated the (), (), () and () expression levels in HLE-B3 cells. Silencing the gene induced EMT of the HLE-B3 cells by promoting the () expression.
Silencing of induces S-phase arrest, also inhibits the proliferation and enhance HLE-B3 apoptosis and EMT, and down-regulates the expression of , , and . Thus, the expression alteration of may play a critical role in the pathogenesis of ARC.
评估（）异常表达在年龄相关性白内障（ARC）发生发展中的调控作用。
采用定量逆转录聚合酶链反应（qRT-PCR）和蛋白质免疫印迹分析评估ARC患者及健康对照者中（）的表达。在（）基因沉默条件下，进一步分析人晶状体上皮细胞（HLE-B3）的增殖、凋亡、细胞周期及上皮-间质转化（EMT）。通过对HLE-B3细胞进行qRT-PCR，评估敲低（）后mRNA水平上基因表达的变化。
（）的异常表达被确定与ARC临床相关。（）沉默通过阻断细胞周期促进HLE-B3细胞凋亡并抑制其增殖。此外，（）基因沉默不影响HLE-B3细胞的细胞骨架，但下调HLE-B3细胞中（）、（）、（）和（）的表达水平。沉默（）基因通过促进（）表达诱导HLE-B3细胞发生EMT。
（）沉默诱导S期阻滞，抑制HLE-B3细胞增殖，增强其凋亡及EMT，并下调（）、（）、（）和（）的表达。因此，（）表达改变可能在ARC发病机制中起关键作用。
