玻璃体内注射卡托普利对小鼠氧诱导性视网膜病变的疗效。
Efficacy of intravitreal captopril on oxygen-induced retinopathy in mice.
作者信息
Di Yu, Zhang Yi-Ou, Yang Yang, Wang Ai-Yuan, Lu Yan, Chen Xiao-Long
机构信息
Department of Ophthalmology, Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning Province, China.
出版信息
Int J Ophthalmol. 2011;4(4):361-4. doi: 10.3980/j.issn.2222-3959.2011.04.07. Epub 2011 Aug 18.
AIM
To study the inhibitory effect of intravitreal captopril on oxygen-induced retinopathy (OIR) in mice.
METHODS
Eighty postnatal day (P)7 C57BL/6J mice were randomly divided into treated group and control group with forty mice in each group. The mice were exposed to 75% ± 2% oxygen for 5 days (P7-P11) and then returned to room air for 5 days (P12-P17) to induce retinal neovascularization (RNV). Beginning on P12, the mice in treated group received daily intravitreal injections of captopril (3.0mL/kg), while those in control group received daily intravitreal injections of phosphate-buffered saline (PBS) (3.0mL/kg) through P17. After anesthetized at P17, one eye was chosen randomly as experimental eye and were enucleated. RNV was examined by Adenosine diphosphate-ase (ADPase) stained retina flat-mounts and was quantitated histologically by counting the neovascular endothelial cell nuclei anterior to inner limiting membrane (ILM). The expressions of matrix metalloproteinase-2 (MMP-2) and vascular endothelial growth factor (VEGF) were measured by immunohistochemical method.
RESULTS
Comparing with control group, more regular distributions, better branch and reduced density of RNV were observed in eyes of treated group. The number of neovascular cell nuclei was less in treated group than that in control group (t=6.135, P<0.01). Stain of MMP-2 and VEGF was weaker in treated group than that in control group.
CONCLUSION
The results indicate that captopril can significantly inhibit RNV in OIR mice.
目的
研究玻璃体内注射卡托普利对小鼠氧诱导性视网膜病变（OIR）的抑制作用。
方法
80只出生后第7天（P7）的C57BL/6J小鼠随机分为治疗组和对照组，每组40只。将小鼠置于75%±2%氧气环境中5天（P7 - P11），然后放回正常空气环境中5天（P12 - P17）以诱导视网膜新生血管形成（RNV）。从P12开始，治疗组小鼠每天玻璃体内注射卡托普利（3.0mL/kg），而对照组小鼠每天玻璃体内注射磷酸盐缓冲盐水（PBS）（3.0mL/kg），持续至P17。在P17麻醉后，随机选择一只眼睛作为实验眼并摘除眼球。通过二磷酸腺苷酶（ADPase）染色的视网膜铺片检查RNV，并通过计数内界膜（ILM）前方的新生血管内皮细胞核进行组织学定量分析。采用免疫组织化学方法检测基质金属蛋白酶-2（MMP-2）和血管内皮生长因子（VEGF）的表达。
结果
与对照组相比，治疗组小鼠眼睛的RNV分布更规则，分支更好且密度降低。治疗组新生血管细胞核数量少于对照组（t = 6.135，P < 0.01）。治疗组MMP-2和VEGF的染色比对照组弱。
结论
结果表明卡托普利可显著抑制OIR小鼠的RNV。
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