Zhu Jiang-Li, Gao Xin-Rui, Cui Hong-Ping, Lang Li-Li, Li Qian, Liao Xin
Shanghai 10 People's Hospital Affiliated to Tongji University School of Medicine, Shanghai 200072, China.
Int J Ophthalmol. 2011;4(4):371-6. doi: 10.3980/j.issn.2222-3959.2011.04.09. Epub 2011 Aug 18.
To establish a repeatable rat model of Fusarium solani keratitis (F. solani keratitis) that mimicked fungal keratitis in humans.
Wistar rats' corneas were scratched on the superficial stroma after scraping the unilateral corneal epithelia. Then, the corneal surface was inoculated with different inoculum dose of F. solani spore suspension. Doses ranged from 10(6) to 10(9) colony-forming unit per milliliter (CFU/mL). The treated corneas were covered by contact lenses that were made of Parafilm M membrane. Negative controls were inoculated with sterile phosphate-buffered saline (PBS). For statistical analysis, corneas were evaluated daily on a 12-point scale to check the state of corneal inflammation. Furthermore, the pathological characteristics of this model were investigated.
The rat model of F. solani keratitis was established by the combination methods of corneal trauma and parafilm-made contact lens and inoculation of fungus spore suspension. 10(6) and 10(7)CFU/mL of F. solani induced mild corneal infection, while 10(8)CFU/mL of F. solani was sufficient to induce moderate infection that was consistent with human keratomycosis. Dose of 10(9)CFU/mL of F. solani was excessive and led to perforated corneas.
The rat model of F. solani keratitis, established by the combinational methods of corneal trauma, parafilm-made contact lens and the appropriate dose of inoculum, that imitates the developing processes of F. solani keratitis in human beings and provides a repeatable method of creating a rat model.
建立一种可重复的茄病镰刀菌角膜炎（茄病镰刀菌性角膜炎）大鼠模型，该模型可模拟人类真菌性角膜炎。
刮除Wistar大鼠单侧角膜上皮后，在浅层基质上划痕。然后，用不同接种剂量的茄病镰刀菌孢子悬浮液接种角膜表面。剂量范围为每毫升10⁶至10⁹菌落形成单位（CFU/mL）。处理后的角膜用Parafilm M膜制成的隐形眼镜覆盖。阴性对照接种无菌磷酸盐缓冲盐水（PBS）。为进行统计分析，每天以12分制评估角膜，以检查角膜炎症状态。此外，还研究了该模型的病理特征。
通过角膜创伤、Parafilm制成的隐形眼镜和真菌孢子悬浮液接种的联合方法建立了茄病镰刀菌角膜炎大鼠模型。10⁶和10⁷CFU/mL的茄病镰刀菌引起轻度角膜感染，而10⁸CFU/mL的茄病镰刀菌足以引起中度感染，这与人类角膜真菌病一致。10⁹CFU/mL的茄病镰刀菌剂量过大，导致角膜穿孔。
通过角膜创伤、Parafilm制成的隐形眼镜和适当接种剂量的联合方法建立的茄病镰刀菌角膜炎大鼠模型，模仿了人类茄病镰刀菌角膜炎的发展过程，并提供了一种可重复创建大鼠模型的方法。
