O'Day D M, Head W S, Robinson R D, Yang R, Shetlar D, Wang M X
Department of Ophthalmology and Visual Sciences, Vanderbilt University School of Medicine, Nashville, Tennessee, USA.
Invest Ophthalmol Vis Sci. 1999 Jun;40(7):1607-11.
A model of experimental keratomycosis was established that mimics human disease in which the only fungi present are those that are actively growing within the cornea.
Dutch-belted rabbits received a subconjunctival injection of triamcinolone acetonide to one eye. One day later the epithelium was removed from the central cornea and a standardized inoculum of Candida albicans blastoconidia was placed on the corneal surface and covered with a contact lens. The lids were closed with a lateral tarsorrhaphy. After 24 hours, the lid sutures and contact lens were removed. Five days later the animals were killed, and their corneas were subjected to separate isolate recovery and histology studies. A group of similarly infected rabbits without corticosteroid injection served as controls.
Both groups developed invasive corneal disease. Although isolate recovery was not significantly different from corticosteroid-treated rabbits compared with controls, fungal biomass was increased. Hyphal invasion was limited to the anterior cornea in control eyes, but penetrated deep stroma in most of the corticosteroid-treated rabbits.
Invasive corneal disease can be established with a surface inoculum. Corticosteroid administration increased corneal penetration of hyphae. Quantitative isolate recovery is not a reliable measure of the fungal load within the cornea.
建立一种实验性角膜真菌病模型，该模型可模拟人类疾病，其中仅存在那些在角膜内活跃生长的真菌。
给荷兰带兔的一只眼睛进行结膜下注射曲安奈德。一天后，从中央角膜去除上皮，将白色念珠菌芽生孢子的标准化接种物置于角膜表面，并用隐形眼镜覆盖。通过外侧睑缘缝合术闭合眼睑。24小时后，拆除眼睑缝线和隐形眼镜。五天后处死动物，对其角膜进行单独的分离菌回收和组织学研究。一组未注射皮质类固醇的类似感染兔子作为对照。
两组均发生侵袭性角膜疾病。虽然与对照组相比，皮质类固醇治疗兔子的分离菌回收率无显著差异，但真菌生物量增加。在对照眼中，菌丝侵袭仅限于角膜前部，但在大多数皮质类固醇治疗的兔子中，菌丝穿透了深层基质。
通过表面接种可建立侵袭性角膜疾病。给予皮质类固醇会增加菌丝在角膜中的穿透。定量分离菌回收不是角膜内真菌负荷的可靠测量方法。
