神经球蛋白在大鼠高眼压诱导的急性缺氧缺血性视网膜损伤中的表达
Expression of neuroglobin in ocular hypertension induced acute hypoxic-ischemic retinal injury in rats.
作者信息
Shi Shao-Yang, Feng Xue-Mei, Li You, Li Xun, Chen Xiao-Long
机构信息
Department of Ophthalmology, Shengjing Hospital of China Medical University, Shenyang 11004, Liaoning Province, China.
出版信息
Int J Ophthalmol. 2011;4(4):393-5. doi: 10.3980/j.issn.2222-3959.2011.04.14. Epub 2011 Aug 18.
AIM
To investigate the expression of neuroglobin (Ngb) in the retina of rats with ocular hypertension induced acute retinal hypoxic-ischemic injury.
METHODS
Seventy Wistar rats were divided into 7 groups randomly. The experimental model was induced by elevation of intraocular pressure via anterior chamber canula insertion in the left eyes and the fellow eyes were preserved as normal controls. The retinal tissues were taken at 1, 5, 10, 15, 20, 30 and 60 minutes after hypoxic-ischemia injury. Protein was extracted, and then analyzed by Western-blot method. SPSS was used for statistical analysis.
RESULTS
The time-depended expressions of Ngb were observed. The level of Ngb increased rapidly at 1 minute after ischemia and reached to the peak at 5 minutes, which had significant difference from that of control group (P<0.05). It kept in high level during 5-15 minutes (P<0.05), then decreased after 20 minutes till 60 minutes. There were no significant differences between experimental and control group in the latter period (P>0.05).
CONCLUSION
The expression of Ngb in retinal tissue increased rapidly after hypoxic-ischemic injury in rats, suggesting that Ngb may play an important role in the process of acute retinal hypoxic-ischemic injury.
目的
研究神经球蛋白（Ngb）在高眼压诱导的大鼠急性视网膜缺氧缺血性损伤模型视网膜中的表达情况。
方法
将70只Wistar大鼠随机分为7组。通过前房插管升高左眼眼压建立实验模型，右眼作为正常对照。在缺氧缺血损伤后1、5、10、15、20、30和60分钟取材视网膜组织，提取蛋白后采用Western-blot法进行分析，用SPSS软件进行统计学分析。
结果
观察到Ngb呈时间依赖性表达。缺血后1分钟Ngb水平迅速升高，5分钟时达到峰值，与对照组相比有显著差异（P<0.05）。在5至15分钟内保持高水平（P<0.05），20分钟后开始下降直至60分钟。后期实验组与对照组之间无显著差异（P>0.05）。
结论
大鼠缺氧缺血性损伤后视网膜组织中Ngb表达迅速增加，提示Ngb可能在急性视网膜缺氧缺血性损伤过程中起重要作用。
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