Li Wan, Che Wen-Jian, Zhang Ming-Chang
Department of Ophthalmology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China.
Int J Ophthalmol. 2011;4(6):582-9. doi: 10.3980/j.issn.2222-3959.2011.06.02. Epub 2011 Dec 18.
To investigate the antifibrotic effect of freeze-dried bilayered fibrin-binding amniotic membrane on trabeculectomy in a rabbit model.
Twenty-four Japanese white rabbits were randomized into three groups: the experimental group (ocular trabeculectomy in combination with freeze-dried bilayered fibrin-binding amniotic membrane transplantation), the control group (ocular trabeculectomy in combination with natural bilayered fibrin-binding amniotic membrane) and the blank group (single trabeculectomy). Clinical observation, hematoxylin-eosin staining, Massion staining, real-time PCR and immunohistochemistry for α-SMA were performed on days 7, 14, 21 and 30 following surgery.
Statistical differences were noted in survival analysis and intraocular pressure (IOP) among groups on days 7, 14, 21 and 30 following surgery. Histology, immunohistochemistry and real-time PCR further demonstrated that trabeculectomy in combination with freeze-dried bilayered fibrin-binding amniotic membrane resulted in good wound healing and no scar formation.
Self-made freeze-dried bilayered fibrin-binding amniotic membrane may inhibit the formation of scarring in glaucoma after trabeculectomy.
在兔模型中研究冻干双层纤维蛋白结合羊膜对小梁切除术的抗纤维化作用。
将24只日本白兔随机分为三组：实验组（小梁切除术联合冻干双层纤维蛋白结合羊膜移植）、对照组（小梁切除术联合天然双层纤维蛋白结合羊膜）和空白组（单纯小梁切除术）。术后第7、14、21和30天进行临床观察、苏木精-伊红染色、Masson染色、实时荧光定量PCR以及α-SMA免疫组织化学检测。
术后第7、14、21和30天，各组间生存分析和眼压（IOP）存在统计学差异。组织学、免疫组织化学和实时荧光定量PCR进一步表明，小梁切除术联合冻干双层纤维蛋白结合羊膜可实现良好的伤口愈合且无瘢痕形成。
自制冻干双层纤维蛋白结合羊膜可能抑制小梁切除术后青光眼瘢痕的形成。
