Shao Yi, Yu Yao, Liu Qiu-Ping, Li Jing-Ming, Dong Fei, Huang Xin, Pei Chong-Gang, Tu Ping, Li Hen-Hui, Gao Gui-Ping
Department of Ophthalmology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China.
Department of Endocrinology, the Third Hospital of Nanchang, the Affiliated Nanchang Hospital of Southern Medical University, Nanchang 330009, Jiangxi Province, China.
Int J Ophthalmol. 2014 Apr 18;7(2):226-31. doi: 10.3980/j.issn.2222-3959.2014.02.07. eCollection 2014.
To investigate the efficacy and safety of Honghua preserved amniotic membrane (AM) for preventing scar formation of the filtering bleb in a rabbit model of glaucoma trabeculectomy surgery.
Totally 36 rabbits (36 eyes) were randomly divided into 3 groups: the experimental group (ocular trabeculectomy in combination with Honghua preserved AM transplantation), the control group (ocular trabeculectomy surgery in combination with AM implantation), and the blank group (single trabeculectomy). Clinical observations [including intraocular pressure (IOP), filtering blebs and complications], Masson-Trichrome staining, real-time quantitative reverse transcription-polymerase chain reaction (real-time PCR), Western blot were performed on different time points (D1, D7, D14, D21 and D56) after the surgery.
After operated for 14d, there were statistically significant differences in the filtering blebs compared to the situation before operation (P<0.05), whereas no statistically difference on that among three groups (P>0.05). After 21d, the IOP of experimental group was lowest (P<0.05). There was significant difference between control group and blank group (P<0.05). On postoperative D14, the mean number of fibroblasts in the experimental group was significantly lower (40.6±10.2) compared to those in the control group (54.4±10.8) and blank group (68.2±11.6) (P<0.05, respectively). The mean numbers of the macrophage in the experimental and control groups were respcitively significantly lower versus the blank group (P<0.05, P<0.05, respectively). Compared to that in blank group, the level of transforming growth factor-β (TGF-β1) expression in sclera and conjunctival areas was reduced in the experimental and control groups on protein and mRNA level (P<0.05), but not significant difference between these two groups (P>0.05).
The trabeculectory surgery with Honghua preserved AM can control IOP, sustain the functional filtration bleb, inhibit the proliferation of fibroblasts and open the filtrating pathway on the rabbit glaucoma models.
在兔青光眼小梁切除术模型中，研究红花保存羊膜（AM）预防滤过泡瘢痕形成的有效性和安全性。
将36只兔（36只眼）随机分为3组：实验组（小梁切除术联合红花保存羊膜移植）、对照组（小梁切除术联合羊膜植入）和空白组（单纯小梁切除术）。在术后不同时间点（第1、7、14、21和56天）进行临床观察[包括眼压（IOP）、滤过泡和并发症]、Masson三色染色、实时定量逆转录聚合酶链反应（实时PCR）、蛋白质印迹法。
术后14天，与术前相比，滤过泡有统计学显著差异（P<0.05），而三组之间无统计学差异（P>0.05）。21天后，实验组眼压最低（P<0.05）。对照组与空白组有显著差异（P<0.05）。术后第14天，实验组成纤维细胞平均数量（40.6±10.2）明显低于对照组（54.4±10.8）和空白组（68.2±11.6）（P均<0.05）。实验组和对照组巨噬细胞平均数量均明显低于空白组（分别为P<0.05，P<0.05）。与空白组相比，实验组和对照组巩膜和结膜区域转化生长因子-β（TGF-β1）在蛋白质和mRNA水平的表达均降低（P<0.05），但两组之间无显著差异（P>0.05）。
在兔青光眼模型中，小梁切除术联合红花保存羊膜可控制眼压，维持功能性滤过泡，抑制成纤维细胞增殖并开放滤过通道。
