The Diabetes Center at Wuhan Central Hospital, No. 26 Shengli Road, Jiangan District, Wuhan, Hubei 430014, China.
Biochem Cell Biol. 2012 Aug;90(4):548-57. doi: 10.1139/o2012-012. Epub 2012 May 3.
We hypothesized that vitamin A (VA) status may affect obesity development. Male Zucker lean (ZL) and fatty (ZF) rats after weaning were fed a synthetic VA deficient (VAD) or VA sufficient (VAS) diet for 8 weeks before their plasma parameters and hepatic genes' expression were analyzed. The body mass (BM) of ZL or ZF rats fed the VAD diet was lower than that of their corresponding controls fed the VAS diet at 5 or 2 weeks, respectively. The VAD ZL and ZF rats had less food intake than the VAS rats after 5 weeks. The VAD ZL and ZF rats had lower plasma glucose, triglyceride, insulin, and leptin levels, as well as lower liver glycogen content, net mass of epididymal fat, and liver/BM and epididymal fat/BM ratios (ZL only) than their respective VAS controls. VAD rats had lower hepatic Cyp26a1, Srebp-1c, Fas, Scd1, Me1, Gck, and Pklr (ZL and ZF); and higher Igfbp1 (ZL and ZF), Pck1(ZF only), and G6pc (ZF only) mRNA levels than their respective VAS controls. We conclude that ZL and ZF rats responded differently to dietary VA deficiency. VA status affected obesity development and altered the expression of hepatic genes for fuel metabolism in ZF rats. The mechanisms will help us to combat metabolic diseases.
我们假设维生素 A (VA) 状态可能影响肥胖的发展。雄性 Zucker 瘦 (ZL) 和肥胖 (ZF) 大鼠断奶后分别喂食合成 VA 缺乏 (VAD) 或 VA 充足 (VAS) 饮食 8 周，然后分析其血浆参数和肝基因表达。与 VAS 饮食组相比，ZL 或 ZF 大鼠在食用 VAD 饮食时，其体重（BM）在第 5 或 2 周时分别较低。与 VAS 组相比，5 周后 VAD ZL 和 ZF 大鼠的食物摄入量减少。VAD ZL 和 ZF 大鼠的血浆葡萄糖、甘油三酯、胰岛素和瘦素水平较低，肝糖原含量、附睾脂肪净质量、肝/BM 和附睾脂肪/BM 比值（仅 ZL）也较低。VAD 大鼠的肝 Cyp26a1、Srebp-1c、Fas、Scd1、Me1、Gck 和 Pklr（ZL 和 ZF）mRNA 水平较低；而 Igfbp1（ZL 和 ZF）、Pck1（仅 ZF）和 G6pc（仅 ZF）的 mRNA 水平较高。我们得出结论，ZL 和 ZF 大鼠对膳食 VA 缺乏的反应不同。VA 状态影响肥胖的发展，并改变了 ZF 大鼠肝基因的燃料代谢表达。这些机制将帮助我们对抗代谢性疾病。
