Aquatic Animal Nutrition and Health Research Group, School of Biological and Biomedical Sciences, University of Plymouth, Plymouth, Devon, UK.
Fish Shellfish Immunol. 2012 Jun;32(6):1002-7. doi: 10.1016/j.fsi.2012.02.020. Epub 2012 Feb 27.
An investigation was conducted to evaluate the effect of feeding a tropical earthworm meal (Perionyx escavatus) on the haemato-immunological response and growth performance of mirror carp (Cyprinus carpio). Fish were fed diets for a total of 88 days, fishmeal served as the main protein source in the control diet. Two remaining diets consisted of fishmeal fixed at 33.65% provision of protein and the remaining 66.35% protein was provided by soybean meal (SBM diet) or P. excavatus meal (EW diet). Compared to control and SBM fed fish (7.69 ± 0.28 and 5.92 ± 0.31 g/dl, respectively), a significant increase in haemoglobin was measured in EW fed fish (9.57 ± 0.24 g/dl). Consequently significant elevations were also observed in mean corpuscular haemoglobin (MCH; 79.13 ± 4.59 pg) and mean corpuscular haemoglobin concentration (MCHC; 22.69 ± 0.54 pg) in EW fed fish. On the contrary, compared to control and SBM fed carp total leukocyte levels (2.72 ± 0.17 and 3.10 ± 0.17 × 10(4)/mm(3), respectively) were significantly decreased in the EW group (2.15 ± 0.14 × 10(4)/mm(3)). Moreover at day 14 and 21 post immunisation with bacterin isolated from Aeromonas hydrophila fish fed the EW diet displayed a significant reduction in respiratory burst activity (RBA) compared to control and SBM fed fish. After 60 days of feeding, fish fed EW diet showed a significant elevation in final body weight compared to fish fed a fishmeal based diet (control treatment) and fish fed a soybean meal based diet. Similar improvements were observed in feed utilisation efficiency. The present study shows that feeding P. excavatus meal to mirror carp decreases some aspects of the innate immune response, but at the same time gives rise to significant enhancement of growth and feed utilisation efficiency.
进行了一项研究，以评估喂养热带蚯蚓粉（Perionyx escavatus）对镜鲤（Cyprinus carpio）的血液免疫反应和生长性能的影响。鱼在饲料中总共喂养了 88 天，鱼粉是对照饮食中的主要蛋白质来源。其余两种饮食由鱼粉组成，其中 33.65%的蛋白质提供蛋白质，其余 66.35%的蛋白质由豆粕（SBM 饮食）或 P. excavatus 粉（EW 饮食）提供。与对照组和 SBM 喂养的鱼（分别为 7.69 ± 0.28 和 5.92 ± 0.31 g/dl）相比，在 EW 喂养的鱼中，血红蛋白显著增加（9.57 ± 0.24 g/dl）。同样，在 EW 喂养的鱼中，平均红细胞血红蛋白（MCH；79.13 ± 4.59 pg）和平均红细胞血红蛋白浓度（MCHC；22.69 ± 0.54 pg）也显著升高。相反，与对照组和 SBM 喂养的鲤鱼相比，总白细胞水平（分别为 2.72 ± 0.17 和 3.10 ± 0.17 × 10（4）/mm（3））在 EW 组中显著降低（2.15 ± 0.14 × 10（4）/mm（3））。此外，在接种了从嗜水气单胞菌中分离的细菌菌苗后 14 天和 21 天，与对照组和 SBM 喂养的鱼相比，喂食 EW 饮食的鱼的呼吸爆发活性（RBA）显著降低。在 60 天的喂养后，与喂食基于鱼粉的饮食（对照处理）和喂食基于豆粕的饮食的鱼相比，喂食 EW 饮食的鱼的最终体重显著增加。在饲料利用效率方面也观察到类似的改善。本研究表明，喂养热带蚯蚓粉会降低镜鲤某些方面的先天免疫反应，但同时会显著提高生长和饲料利用效率。
