Harikrishnan Ramasamy, Balasundaram Chellam, Heo Moon-Soo
Marine and Environmental Research Institute, Marine Applied Microbes and Aquatic Organism Disease Control Laboratory, Department of Aquatic Biomedical Sciences, School of Marine Biomedical Sciences, College of Ocean Sciences, Jeju National University, Jeju 690-756, South Korea.
Dis Aquat Organ. 2010 Oct 26;92(1):41-9. doi: 10.3354/dao02240.
This study reports the effect of probiotic- and triherbal extract-enriched diet on Cyprinus carpio (52 +/- 2 g, n = 250) inoculated intramuscularly (50 microl) with Aeromonas hydrophila (1.8 x 106 CFU ml(-1)). On Day 6 post-infection, the fish were divided into 4 groups and fed with the basal diet (IU) or with diets supplemented with triherbal extract (H), Sporolac (S) or Lactobacillus (L). A fifth group of non-infected fish fed with the basal diet was used as a control. Haematology and immunology parameters were measured in Weeks 1, 2 and 4. In the H, S, and L groups, white blood cell levels were significantly increased (p < 0.05) throughout the experimental period. In the H and S groups, red blood cell and haemoglobin levels decreased significantly (p < 0.05) in Weeks 1, 2 and 4. In all the diet groups, the haematocrit and mean corpuscular volume did not differ significantly (p > 0.05) in Week 1, and these values remained near those of the control in Week 2. The S and L groups did not exhibit significant changes (p > 0.05) in glucose, and cholesterol levels in Weeks 2 and 4 compared to controls, and the L group also showed no change in total protein. Respiratory burst activity was significantly increased in Weeks 1 to 4 in the H, S and L groups. Serum-mediated killing of Escherichia coli was significantly increased in the L group in Weeks 1 to 4. Mortalities after A. hydrophila challenge were higher in the IU (85%) and H (50%) groups than in the S (45%) and L (35%) groups. Our results show that formulated probiotic-enriched diets can speed up the recovery of ulcerative dermatitis induced by A. hydrophila in carp.
本研究报告了富含益生菌和三草药提取物的饲料对体重为52±2克（n = 250）的鲤鱼的影响，这些鲤鱼通过肌肉注射（50微升）接种了嗜水气单胞菌（1.8×106 CFU ml(-1)）。在感染后第6天，将鱼分为4组，分别投喂基础饲料（IU）或添加了三草药提取物（H）、芽孢杆菌制剂（S）或乳酸杆菌（L）的饲料。第五组未感染且投喂基础饲料的鱼用作对照。在第1、2和4周测量血液学和免疫学参数。在H、S和L组中，整个实验期间白细胞水平显著升高（p < 0.05）。在H和S组中，第1、2和4周红细胞和血红蛋白水平显著下降（p < 0.05）。在所有饲料组中，第1周血细胞比容和平均红细胞体积无显著差异（p > 0.05），第2周这些值仍接近对照组。与对照组相比，S和L组在第2和4周的葡萄糖和胆固醇水平无显著变化（p > 0.05），L组总蛋白也无变化。在第1至4周，H、S和L组的呼吸爆发活性显著增加。在第1至4周，L组血清介导的大肠杆菌杀伤作用显著增强。嗜水气单胞菌攻击后的死亡率在IU组（85%）和H组（50%）高于S组（45%）和L组（35%）。我们的结果表明，配制的富含益生菌的饲料可加速鲤鱼因嗜水气单胞菌引起的溃疡性皮肤病的恢复。
