多瘤病毒DNA在分离细胞核中的合成：复制叉缺陷的证据
Polyoma DNA synthesis in isolated nuclei: evidence for defective replication forks.
作者信息
Bjursell G, Munck V, Therkelsen A J
出版信息
J Virol. 1979 Jun;30(3):929-32. doi: 10.1128/JVI.30.3.929-932.1979.
Abstract
Nuclei, isolated from polyoma virus-infected mouse 3T6 cells, were incubated under condtions suitable for polyoma DNA synthesis. By using electrom microscopy and standard regression statistics, it was shown that replication is mainly unidirectional in a large number of molecules, indicating the presence of inactive replication forks. The replication forks were inactivated randomly, and the defect seemed to be present from the beginning of the in vitro incubation.
摘要
从多瘤病毒感染的小鼠3T6细胞中分离出的细胞核，在适合多瘤病毒DNA合成的条件下进行孵育。通过电子显微镜和标准回归统计分析表明，在大量分子中复制主要是单向的，这表明存在无活性的复制叉。复制叉随机失活，而且这种缺陷似乎在体外孵育开始时就已存在。
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5
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