多瘤病毒DNA在分离细胞核中的合成：复制叉缺陷的证据
Polyoma DNA synthesis in isolated nuclei: evidence for defective replication forks.
作者信息
Bjursell G, Munck V, Therkelsen A J
出版信息
J Virol. 1979 Jun;30(3):929-32. doi: 10.1128/JVI.30.3.929-932.1979.
Abstract
Nuclei, isolated from polyoma virus-infected mouse 3T6 cells, were incubated under condtions suitable for polyoma DNA synthesis. By using electrom microscopy and standard regression statistics, it was shown that replication is mainly unidirectional in a large number of molecules, indicating the presence of inactive replication forks. The replication forks were inactivated randomly, and the defect seemed to be present from the beginning of the in vitro incubation.
摘要
从多瘤病毒感染的小鼠3T6细胞中分离出的细胞核，在适合多瘤病毒DNA合成的条件下进行孵育。通过电子显微镜和标准回归统计分析表明，在大量分子中复制主要是单向的，这表明存在无活性的复制叉。复制叉随机失活，而且这种缺陷似乎在体外孵育开始时就已存在。
相似文献
1
Polyoma DNA synthesis in isolated nuclei: evidence for defective replication forks.
J Virol. 1979 Jun;30(3):929-32. doi: 10.1128/JVI.30.3.929-932.1979.
2
Replication of polyoma DNA in isolated nuclei: analysis of replication fork movement.
J Virol. 1979 Nov;32(2):386-93. doi: 10.1128/JVI.32.2.386-393.1979.
3
Hydroxyurea-induced accumulation of short fragments during polyoma DNA replication. II. Behavior during incubation of isolated nuclei.
J Virol. 1973 Sep;12(3):609-15. doi: 10.1128/JVI.12.3.609-615.1973.
4
Replication of polyoma DNA in isolated nuclei. V. Complementation of in vitro DNA replication.
J Virol. 1975 Feb;15(2):259-67. doi: 10.1128/JVI.15.2.259-267.1975.
5
Replication of polyoma DNA in isolated nuclei. I. Characterization of the system from mouse fibroblast 3T6 cells.
J Mol Biol. 1972 Dec 30;72(3):523-37. doi: 10.1016/0022-2836(72)90172-6.
6
Replication of polyoma DNA in isolated nuclei. II. Evidence for semi-conservative replication.
J Mol Biol. 1972 Dec 30;72(3):539-52. doi: 10.1016/0022-2836(72)90173-8.
7
Replication of polyoma DNA in isolated nuclei. Synthesis and distribution of initiator RNA.
J Biol Chem. 1978 Oct 25;253(20):7469-75.
8
Hydroxyurea-induced accumulation of short fragments during polyoma DNA replication. I. Characterization of fragments.
J Virol. 1973 Sep;12(3):600-8. doi: 10.1128/JVI.12.3.600-608.1973.
9
Initiation of polyoma virus DNA replication in vitro and its dependence on the viral gene A protein.
Nucleic Acids Res. 1980 Oct 10;8(19):4377-92. doi: 10.1093/nar/8.19.4377.
10
Asynchronous bidirectional replication of polyoma virus DNA.
J Virol. 1982 Sep;43(3):885-95. doi: 10.1128/JVI.43.3.885-895.1982.
引用本文的文献
1
Synthesis of multimeric polyoma virus DNA in mouse L-cells: role of the tsA1S9 gene product.
J Virol. 1983 Jun;46(3):768-77. doi: 10.1128/JVI.46.3.768-777.1983.
2
Asynchronous bidirectional replication of polyoma virus DNA.
J Virol. 1982 Sep;43(3):885-95. doi: 10.1128/JVI.43.3.885-895.1982.
3
Distribution of replicating simian virus 40 DNA in intact cells and its maturation in isolated nuclei.
J Virol. 1982 Mar;41(3):877-92. doi: 10.1128/JVI.41.3.877-892.1982.
本文引用的文献
1
Initiator RNA in discontinuous polyoma DNA synthesis.
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4901-5. doi: 10.1073/pnas.71.12.4901.
2
Location of the origin and terminus of replication in polyoma virus DNA.
J Gen Virol. 1974 Oct;25(1):133-42. doi: 10.1099/0022-1317-25-1-133.
3
Replication of polyoma DNA in isolated nuclei. 3. The nucleotide sequence at the RNA-DNA junction of nascent strands.
J Mol Biol. 1974 Mar 25;84(1):197-216. doi: 10.1016/0022-2836(74)90222-8.
4
Hydroxyurea-induced accumulation of short fragments during polyoma DNA replication. II. Behavior during incubation of isolated nuclei.
J Virol. 1973 Sep;12(3):609-15. doi: 10.1128/JVI.12.3.609-615.1973.
5
Replication of polyoma DNA in isolated nuclei. II. Evidence for semi-conservative replication.
J Mol Biol. 1972 Dec 30;72(3):539-52. doi: 10.1016/0022-2836(72)90173-8.
6
Replication of polyoma DNA in isolated nuclei. I. Characterization of the system from mouse fibroblast 3T6 cells.
J Mol Biol. 1972 Dec 30;72(3):523-37. doi: 10.1016/0022-2836(72)90172-6.
7
Simian virus 40 deoxyribonucleic acid synthesis: analysis by gel electrophoresis.
J Virol. 1972 Oct;10(4):599-604. doi: 10.1128/JVI.10.4.599-604.1972.
8
Origin and direction of simian virus 40 deoxyribonucleic acid replication.
J Virol. 1972 Sep;10(3):484-91. doi: 10.1128/JVI.10.3.484-491.1972.
9
Selective extraction of polyoma DNA from infected mouse cell cultures.
J Mol Biol. 1967 Jun 14;26(2):365-9. doi: 10.1016/0022-2836(67)90307-5.
10
The replication of polyoma DNA.
J Gen Virol. 1973 Dec;21(3):515-21. doi: 10.1099/0022-1317-21-3-515.
Zotero 插件