羟基脲诱导多瘤病毒DNA复制过程中短片段的积累。I. 片段的特征
Hydroxyurea-induced accumulation of short fragments during polyoma DNA replication. I. Characterization of fragments.
作者信息
Magnusson G
出版信息
J Virol. 1973 Sep;12(3):600-8. doi: 10.1128/JVI.12.3.600-608.1973.
Abstract
Hydroxyurea treatment of 3T6 mouse fibroblast cells infected with polyoma virus resulted within 15 min in more than a 20-fold reduction of the rate of both viral and cellular DNA synthesis. After the initial rapid inhibition, the rate of DNA synthesis remained essentially constant for at least 2 h. In the inhibited cells viral DNA accumulated as short chains with a sedimentation coefficient of about 4S (hydroxyurea fragments). A variable proportion of these fragments was released from the template strands when the viral DNA was extracted by the Hirt procedure. Reannealing experiments demonstrated that hydroxyurea fragments were polyoma-specific and probably synthesized on both parental strands at the replication forks.
摘要
用羟基脲处理感染多瘤病毒的3T6小鼠成纤维细胞，15分钟内病毒和细胞DNA合成速率降低了20倍以上。在最初的快速抑制之后，DNA合成速率至少在2小时内基本保持恒定。在受抑制的细胞中，病毒DNA以沉降系数约为4S的短链形式积累（羟基脲片段）。当通过赫特方法提取病毒DNA时，这些片段中有可变比例从模板链上释放出来。复性实验表明，羟基脲片段是多瘤病毒特异性的，可能在复制叉处的两条亲代链上合成。
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