• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

羟基脲诱导多瘤病毒DNA复制过程中短片段的积累。I. 片段的特征

Hydroxyurea-induced accumulation of short fragments during polyoma DNA replication. I. Characterization of fragments.

作者信息

Magnusson G

出版信息

J Virol. 1973 Sep;12(3):600-8. doi: 10.1128/JVI.12.3.600-608.1973.

DOI:10.1128/JVI.12.3.600-608.1973
PMID:4355857
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC356668/
Abstract

Hydroxyurea treatment of 3T6 mouse fibroblast cells infected with polyoma virus resulted within 15 min in more than a 20-fold reduction of the rate of both viral and cellular DNA synthesis. After the initial rapid inhibition, the rate of DNA synthesis remained essentially constant for at least 2 h. In the inhibited cells viral DNA accumulated as short chains with a sedimentation coefficient of about 4S (hydroxyurea fragments). A variable proportion of these fragments was released from the template strands when the viral DNA was extracted by the Hirt procedure. Reannealing experiments demonstrated that hydroxyurea fragments were polyoma-specific and probably synthesized on both parental strands at the replication forks.

摘要

用羟基脲处理感染多瘤病毒的3T6小鼠成纤维细胞，15分钟内病毒和细胞DNA合成速率降低了20倍以上。在最初的快速抑制之后，DNA合成速率至少在2小时内基本保持恒定。在受抑制的细胞中，病毒DNA以沉降系数约为4S的短链形式积累（羟基脲片段）。当通过赫特方法提取病毒DNA时，这些片段中有可变比例从模板链上释放出来。复性实验表明，羟基脲片段是多瘤病毒特异性的，可能在复制叉处的两条亲代链上合成。

相似文献

1
Hydroxyurea-induced accumulation of short fragments during polyoma DNA replication. I. Characterization of fragments.羟基脲诱导多瘤病毒DNA复制过程中短片段的积累。I. 片段的特征
J Virol. 1973 Sep;12(3):600-8. doi: 10.1128/JVI.12.3.600-608.1973.
2
Hydroxyurea-induced accumulation of short fragments during polyoma DNA replication. II. Behavior during incubation of isolated nuclei.羟基脲诱导的多瘤病毒DNA复制过程中短片段的积累。II. 分离细胞核孵育期间的行为
J Virol. 1973 Sep;12(3):609-15. doi: 10.1128/JVI.12.3.609-615.1973.
3
RNA-linked short DNA fragments during polyoma replication.多瘤病毒复制过程中与RNA相连的短DNA片段。
Proc Natl Acad Sci U S A. 1973 Feb;70(2):412-5. doi: 10.1073/pnas.70.2.412.
4
Replication of polyoma DNA in isolated nuclei. I. Characterization of the system from mouse fibroblast 3T6 cells.多瘤病毒DNA在分离细胞核中的复制。I. 来自小鼠成纤维细胞3T6细胞的系统特性
J Mol Biol. 1972 Dec 30;72(3):523-37. doi: 10.1016/0022-2836(72)90172-6.
5
Replication of polyoma DNA in isolated nuclei. V. Complementation of in vitro DNA replication.多瘤病毒DNA在分离细胞核中的复制。V. 体外DNA复制的互补作用。
J Virol. 1975 Feb;15(2):259-67. doi: 10.1128/JVI.15.2.259-267.1975.
6
Replication of polyoma DNA: effects of hydroxyurea and arabinosyl cytosine.多瘤病毒DNA的复制：羟基脲和阿糖胞苷的作用
Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:227-33. doi: 10.1101/sqb.1974.039.01.029.
7
The replication of the ring-shaped DNA of polyoma virus. II. Identification of molecules at various stages of replication.多瘤病毒环状DNA的复制。II. 复制各阶段分子的鉴定。
J Mol Biol. 1971 Jul 14;59(1):195-206. doi: 10.1016/0022-2836(71)90421-9.
8
The effect of cycloheximide on DNA synthesis in cells productively-infected with polyoma virus.放线菌酮对多瘤病毒有效感染细胞中DNA合成的影响。
Virology. 1970 Dec;42(4):979-92. doi: 10.1016/0042-6822(70)90346-6.
9
Polyoma gene function required for viral DNA synthesis.病毒DNA合成所需的多瘤病毒基因功能。
Virology. 1973 Sep;55(1):127-35. doi: 10.1016/s0042-6822(73)81014-1.
10
Polyoma pseudovirions. I. Sequence of events in primary mouse embryo cells leading to pseudovirus production.多瘤假病毒颗粒。I. 原代小鼠胚胎细胞中导致假病毒产生的事件序列。
J Virol. 1972 Sep;10(3):340-6. doi: 10.1128/JVI.10.3.340-346.1972.

引用本文的文献

1
CDK-mediated phosphorylation of PNKP is required for end-processing of single-strand DNA gaps on Okazaki fragments and genome stability.CDK介导的PNKP磷酸化是冈崎片段上单链DNA缺口的末端加工和基因组稳定性所必需的。
Elife. 2025 Mar 27;14:e99217. doi: 10.7554/eLife.99217.
2
The Importance of Poly(ADP-Ribose) Polymerase as a Sensor of Unligated Okazaki Fragments during DNA Replication.聚（ADP-核糖）聚合酶作为 DNA 复制过程中未连接的冈崎片段传感器的重要性。
Mol Cell. 2018 Jul 19;71(2):319-331.e3. doi: 10.1016/j.molcel.2018.06.004. Epub 2018 Jul 5.
3
Inhibition of DNA chain elongation in a purine-auxotrophic mutant of Chinese hamster.中国仓鼠嘌呤营养缺陷型突变体中DNA链延伸的抑制作用。
J Cell Biol. 1980 Jun;85(3):777-85. doi: 10.1083/jcb.85.3.777.
4
Reduced rate of DNA replication fork movement in megaloblastic anemia.巨幼细胞贫血中DNA复制叉移动速率降低。
J Clin Invest. 1980 Jan;65(1):26-36. doi: 10.1172/JCI109656.
5
Replication of colicinogenic factor E 1 DNA: evidence for a discontinuous replication mechanism.产大肠杆菌素因子E1 DNA的复制：关于不连续复制机制的证据
Nucleic Acids Res. 1974 Sep;1(9):1153-64. doi: 10.1093/nar/1.9.1153.
6
Synthesis of simian virus 40 DNA in isolated nuclei.在分离的细胞核中合成猴病毒40 DNA。
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1045-9. doi: 10.1073/pnas.71.4.1045.
7
Hydroxyurea-induced accumulation of short fragments during polyoma DNA replication. II. Behavior during incubation of isolated nuclei.羟基脲诱导的多瘤病毒DNA复制过程中短片段的积累。II. 分离细胞核孵育期间的行为
J Virol. 1973 Sep;12(3):609-15. doi: 10.1128/JVI.12.3.609-615.1973.
8
Adenovirus type 2 DNA replication. I. Evidence for discontinuous DNA synthesis.腺病毒2型DNA复制。I. 间断性DNA合成的证据。
J Virol. 1975 Apr;15(4):744-58. doi: 10.1128/JVI.15.4.744-758.1975.
9
Evidence for RNA linked to nascent DNA in HeLa cells.在HeLa细胞中RNA与新生DNA相关的证据。
J Cell Biol. 1976 Mar;68(3):557-66. doi: 10.1083/jcb.68.3.557.
10
Replication of polyoma DNA in isolated nuclei: analysis of replication fork movement.多瘤病毒DNA在分离细胞核中的复制：复制叉移动分析
J Virol. 1979 Nov;32(2):386-93. doi: 10.1128/JVI.32.2.386-393.1979.

本文引用的文献

1
THE DEOXYRIBONUCLEASES OF ESCHERICHIA COLI. V. ON THE SPECIFICITY OF EXONUCLEASE I (PHOSPHODIESTERASE).大肠杆菌的脱氧核糖核酸酶。V. 关于核酸外切酶I（磷酸二酯酶）的特异性
J Biol Chem. 1964 Aug;239:2628-36.
2
HYDROXYUREA: INHIBITORY EFFECT ON DNA METABOLISM.羟基脲：对DNA代谢的抑制作用。
Science. 1964 Nov 27;146(3648):1172-4. doi: 10.1126/science.146.3648.1172.
3
A DEOXYRIBONUCLEIC ACID PHOSPHATASE-EXONUCLEASE FROM ESCHERICHIA COLI. II. CHARACTERIZATION OF THE EXONUCLEASE ACTIVITY.来自大肠杆菌的一种脱氧核糖核酸磷酸酶-核酸外切酶。II. 核酸外切酶活性的特性
J Biol Chem. 1964 Jan;239:251-8.
4
A DEOXYRIBONUCLEIC ACID PHOSPHATASE-EXONUCLEASE FROM ESCHERICHIA COLI. I. PURIFICATION OF THE ENZYME AND CHARACTERIZATION OF THE PHOSPHATASE ACTIVITY.来自大肠杆菌的一种脱氧核糖核酸磷酸酶-核酸外切酶。I. 酶的纯化及磷酸酶活性的表征
J Biol Chem. 1964 Jan;239:242-50.
5
Effects of hydroxyurea and 1-beta-D-arabinofuranosyl-cytosine on deoxyribonucleotide pools in mouse embryo cells.羟基脲和1-β-D-阿拉伯呋喃糖基胞嘧啶对小鼠胚胎细胞中脱氧核苷酸库的影响。
Eur J Biochem. 1971 Mar 1;19(1):81-9. doi: 10.1111/j.1432-1033.1971.tb01290.x.
6
Studies of DNA replication in vivo. I. Isolation of the first intermediate of DNA replication in bacteria as single-stranded DNA.体内DNA复制的研究。I. 细菌中DNA复制首个中间体作为单链DNA的分离。
Proc Natl Acad Sci U S A. 1968 May;60(1):329-36. doi: 10.1073/pnas.60.1.329.
7
Mechanism of DNA chain growth. I. Possible discontinuity and unusual secondary structure of newly synthesized chains.DNA链生长的机制。I. 新合成链可能的不连续性和异常二级结构。
Proc Natl Acad Sci U S A. 1968 Feb;59(2):598-605. doi: 10.1073/pnas.59.2.598.
8
The effects of ferrous ion and dithioerythritol on inhibition by hydroxyruea of ribonucleotide reductase.亚铁离子和二硫苏糖醇对羟基脲抑制核糖核苷酸还原酶的影响。
Cancer Res. 1969 Feb;29(2):291-5.
9
Inhibition of ribonucleoside diphosphate reductase by hydroxyurea.羟基脲对核糖核苷二磷酸还原酶的抑制作用。
Cancer Res. 1968 Aug;28(8):1559-65.
10
RNA-linked nascent DNA fragments in Escherichia coli.大肠杆菌中与RNA相连的新生DNA片段。
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1863-7. doi: 10.1073/pnas.69.7.1863.