Departamento de Biología Funcional (Área de Fisiología), Facultad de Medicina, Universidad de Oviedo, C/Julián Claveria 6, E-33006 Oviedo, Spain.
Neurosci Lett. 2012 May 31;517(2):113-7. doi: 10.1016/j.neulet.2012.04.039. Epub 2012 Apr 23.
The purpose of this study was to examine whether progressive training exercise resulted in changes in neuronal expression of c-Fos in the hypothalamic regions (paraventricular nucleus, supraoptic nucleus and suprachiasmatic nucleus) and subfornical organ of Wistar rats and its relation to hydromineral parameters such as plasma proteins, osmolality and hematocrit. Rats were trained progressively in a running wheel over four days, while control rats were not provided with the opportunity to exercise. c-Fos cellular activity was immunohistochemically stained in accordance with the ABC method. The number of c-Fos immunoreactive cells was counted using standard imaging software. c-Fos in the PVN and SO nuclei was found to be significantly increased in trained rats 1h post-exercise compared with control and 24h post-exercise groups. However, no significant differences were found between trained and control rats in the SQ and SFO. These findings provide useful information of interest for future studies on brain specific regions involved in hydromineral balance in response to progressive exercise.
本研究旨在探讨渐进式训练运动是否会导致 Wistar 大鼠下丘脑区域（室旁核、视上核和视交叉上核）和穹窿下器官神经元表达 c-Fos 的变化，以及其与血浆蛋白、渗透压和红细胞压积等水盐参数的关系。大鼠在跑步轮上进行了为期四天的渐进式训练，而对照组大鼠则没有机会进行运动。采用 ABC 法对 c-Fos 细胞活性进行免疫组织化学染色。使用标准成像软件对 c-Fos 免疫反应性细胞的数量进行计数。与对照组和运动后 24 小时组相比，运动后 1 小时训练组的 PVN 和 SO 核中的 c-Fos 明显增加。然而，在 SQ 和 SFO 中，训练组和对照组之间没有发现显著差异。这些发现为未来研究与渐进式运动反应相关的特定于大脑的水盐平衡区域提供了有用的信息。
