Department of Chemistry, Materials and Chemical Engineering Giulio Natta, Politecnico di Milano, Via Mancinelli 7, Milan, Italy.
J Proteomics. 2012 Jun 18;75(11):3374-9. doi: 10.1016/j.jprot.2012.04.020. Epub 2012 Apr 24.
The trace proteome of a Braulio aperitif (a 21% alcohol beverage, named after a mountain in the Val di Stelvio, Italy) has been investigated via capture with combinatorial peptide ligand libraries (CPLL, ProteoMiner). This aperitif is made with an infusion of 13 mountain herbs and berries, among which four are officially indicated in the label: Achillea moschata, juniper (Juniperus communis subsp. alpina) berries, absinthe (Artemisia absinthium) and gentian (Gentiana alpina) roots. Via capture with CPLLs at pH 7.0 and 2.2 we were able to identify 29 unique gene products, among which the PR5 (parasite resistance) allergen Jun r 3.2, a 25kDa species from Juniperus rigida. Due to the paucity of data on these alpine herbs, it was difficult to attribute these proteins to the specific plant extracts presumably present in this beverage; however most of the species identified indeed belong to alpine herbs and plants, living in a habitat between 1000 and 2000m of elevation. Most of them are enzymes, spanning a Mr range from 10 to 65kDa. It is hoped that such a proteomic signature should help tracking counterfeited products sold on the market.
对一款名为 Braulio 的开胃酒（酒精度 21%，得名于意大利的斯泰尔维奥山）的痕量蛋白质组进行了研究，该酒由 13 种高山草药和浆果制成，其中有 4 种在标签上有明确标注：山艾（Achillea moschata）、杜松（Juniperus communis subsp. alpina）浆果、苦艾（Artemisia absinthium）和龙胆（Gentiana alpina）根。通过在 pH 值为 7.0 和 2.2 时用组合肽配体文库（CPLL，ProteoMiner）进行捕获，我们鉴定出了 29 种独特的基因产物，其中包括 PR5（寄生虫抗性）过敏原 Jun r 3.2，这是一种来自 Juniperus rigida 的 25kDa 物种。由于这些高山草药的数据很少，因此很难将这些蛋白质归因于推测存在于这种饮料中的特定植物提取物；然而，鉴定出的大多数物种确实属于高山草药和植物，生活在海拔 1000 至 2000 米之间的栖息地。它们大多是酶，分子量范围从 10 到 65kDa。希望这样的蛋白质组特征有助于追踪市场上销售的假冒产品。
