Department of Chemical and Materials Engineering, National Yunlin University of Science and Technology, Yunlin, Taiwan.
J Proteomics. 2012 Jun 27;75(12):3617-29. doi: 10.1016/j.jprot.2012.04.017. Epub 2012 Apr 21.
Alzheimer's disease (AD) is the most common cause of dementia of late life. To enhance our understanding of AD proteome, the serum proteins were analyzed using two-dimensional gel electrophoresis (2DE) combined with nano-high performance liquid chromatography electrospray ionization tandem mass spectrometry (nano-HPLC-ESI-MS/MS) followed by peptide fragmentation patterning. In this study, six protein spots with differential expression were identified. Five up-regulated proteins were identified as actin, apolipoprotein A-IV (Apo A-IV), inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), alpha-1-antitrypsin (AAT), and antithrombin-III (AT-III); one protein, activity-dependent neuroprotector homeobox protein (ADNP) was down-regulated in AD patients. These proteins with differential expression in the serum may serve as potential indicators of AD. Our results suggested that ADNP may play an important role in slowing the progression of clinical symptoms of AD.
阿尔茨海默病（AD）是老年期痴呆最常见的病因。为了提高我们对 AD 蛋白质组的认识，采用二维凝胶电泳（2DE）联合纳升级高效液相色谱-电喷雾离子串联质谱（nano-HPLC-ESI-MS/MS）分析了血清蛋白，然后对肽段进行了碎片模式分析。在这项研究中，鉴定出了 6 个具有差异表达的蛋白点。5 个上调的蛋白分别被鉴定为肌动蛋白、载脂蛋白 A-IV（Apo A-IV）、α1-抗胰蛋白酶重链 H4（ITIH4）、α1-抗胰蛋白酶（AAT）和抗凝血酶-III（AT-III）；1 个下调的蛋白为活性依赖性神经营养因子同源框蛋白（ADNP）。这些在血清中具有差异表达的蛋白质可能作为 AD 的潜在标志物。我们的结果表明，ADNP 可能在减缓 AD 临床症状的进展中发挥重要作用。
